The Effectof CalciumHydroxideon FibroblastCellsViability
Calcium hydroxide [Ca(OH)2]is widely used as medicament in dental pulp and root canal therapy. Previous' studies demonstrate the aoility of calcium hydroxide to induce necrosis in dental pulp tissue. However, the mechanism of tissue destruction remains unknown. The aim of this study was to inve...
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[Yogyakarta] : Universitas Gadjah Mada
2011
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| Summary: | Calcium hydroxide [Ca(OH)2]is widely used as medicament in dental pulp and root canal therapy. Previous' studies demonstrate the aoility of calcium hydroxide to induce necrosis in dental pulp tissue. However, the mechanism of tissue destruction remains unknown. The aim of this study was to investigate fibroblast cell viability in response to calcium hydroxide exposure. In this study, Vero fibroblast cell line was treated with various concentrations of calcium hydroxide for 24 hours. Cellviabilitywas measured by using MTTassay. Our results showed significantdecrease in cellviabilityafter exposed with calcium hydroxide at concentration 62.5 and 125 Jlg/ml. The result indicated that calcium hydroxideinduced cell death in Verocell line in a dosedependent manner. This study suggests that fibroblast cell death may involved in the mechanism of pulp tissue necrosis caused by calcium hydroxide. |
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