ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA
Psidium guajava L. (Myrtaceae) is a well known plant in Malaysia and Indonesia. Its leaves extract was found to possess antidiarrhea, antimicrobial, hepatoprotective and antioxidant activities. Objective of this research is to isolate an antioxidant subtance from Red Psidium guajava L. leaves. The c...
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[Yogyakarta] : Universitas Gadjah Mada
2012
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| author | Fidrianny, Irda Hartati, Rika Raveendaran, Narmmatha |
| author_facet | Fidrianny, Irda Hartati, Rika Raveendaran, Narmmatha |
| author_sort | Fidrianny, Irda |
| collection | UGM |
| description | Psidium guajava L. (Myrtaceae) is a well known plant in Malaysia and Indonesia. Its leaves extract was found to possess antidiarrhea, antimicrobial, hepatoprotective and antioxidant activities. Objective of this research is to isolate an antioxidant subtance from Red Psidium guajava L. leaves. The crude leaves was extracted using Soxhlet apparatus by gradual polarity of three different solvents, n-hexane, ethyl acetate and methanol. Antioxidant activity of each extract was tested by using DPPH(2,2- diphenyl-1-picrylhydrazyl) radical scavenging method. Total phenol, total flavonoid and total tannin content of the extracts were also measured. Ethyl acetate extract was fractionated using vacuum liquid chromato-graphy for fractionation. Purification was performed using TLC preparativ. Isolate then characterizedusing specific spray reagent, UV-Vis spectrophotometry and infrared spectrophotometry. Crude drug of Psidiumguajava contained flavonoid, tannin, quinone, saponin and steroid/ triterpenoid. Antioxidant activity of ethyl acetate extract is 65.63% with total phenol 4.25%, total flavonoid 0.53% and total tannin 1.16%. Antioxidant compound N was isolated from ethyl acetate extract. Antioxidant compound N was supposed to be aglycone flavone that has OH at C -4', C-5 and C-7. |
| first_indexed | 2024-03-05T23:13:37Z |
| format | Article |
| id | oai:generic.eprints.org:29158 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:09:44Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:291582016-01-25T02:01:16Z https://repository.ugm.ac.id/29158/ ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA Fidrianny, Irda Hartati, Rika Raveendaran, Narmmatha Pharmacology and Pharmaceutical Sciences Psidium guajava L. (Myrtaceae) is a well known plant in Malaysia and Indonesia. Its leaves extract was found to possess antidiarrhea, antimicrobial, hepatoprotective and antioxidant activities. Objective of this research is to isolate an antioxidant subtance from Red Psidium guajava L. leaves. The crude leaves was extracted using Soxhlet apparatus by gradual polarity of three different solvents, n-hexane, ethyl acetate and methanol. Antioxidant activity of each extract was tested by using DPPH(2,2- diphenyl-1-picrylhydrazyl) radical scavenging method. Total phenol, total flavonoid and total tannin content of the extracts were also measured. Ethyl acetate extract was fractionated using vacuum liquid chromato-graphy for fractionation. Purification was performed using TLC preparativ. Isolate then characterizedusing specific spray reagent, UV-Vis spectrophotometry and infrared spectrophotometry. Crude drug of Psidiumguajava contained flavonoid, tannin, quinone, saponin and steroid/ triterpenoid. Antioxidant activity of ethyl acetate extract is 65.63% with total phenol 4.25%, total flavonoid 0.53% and total tannin 1.16%. Antioxidant compound N was isolated from ethyl acetate extract. Antioxidant compound N was supposed to be aglycone flavone that has OH at C -4', C-5 and C-7. [Yogyakarta] : Universitas Gadjah Mada 2012 Article NonPeerReviewed Fidrianny, Irda and Hartati, Rika and Raveendaran, Narmmatha (2012) ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA. Indonesian Journal of Pharmacy, 23 (1). pp. 36-40. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12221 |
| spellingShingle | Pharmacology and Pharmaceutical Sciences Fidrianny, Irda Hartati, Rika Raveendaran, Narmmatha ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA |
| title | ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA |
| title_full | ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA |
| title_fullStr | ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA |
| title_full_unstemmed | ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA |
| title_short | ANTIOXIDANT ACTIVITY OF ETHYLACETATE EXTRACTOF RED Psidium guajava L. LEAVES GROWN IN MANOKO, LEMBANG- INDONESIA |
| title_sort | antioxidant activity of ethylacetate extractof red psidium guajava l leaves grown in manoko lembang indonesia |
| topic | Pharmacology and Pharmaceutical Sciences |
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