Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus

A panel of six monoclonal antibodies against the nucleocapsid protein (NP) of Newcastle disease virus (NDV) strain AF2240 was produced by immunization of Balb/c mice with purified NP. Isotyping test revealed three of the monoclonal (mAbs) were IgG1 and the others were IgG2a. All of the mAbs recog...

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Main Authors: Ahmad Raus, Raha, Ali, Abdul Manaf, Tan, Wen Siang, Mohd. Salleh, Hamzah, Eshagi, Majid, Yusoff, Khatijah
Format: Proceeding Paper
Language:English
Published: 2007
Subjects:
Online Access:http://irep.iium.edu.my/29278/1/ICBioE_Paper_raha1%27_2007.pdf
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author Ahmad Raus, Raha
Ali, Abdul Manaf
Tan, Wen Siang
Mohd. Salleh, Hamzah
Eshagi, Majid
Yusoff, Khatijah
author_facet Ahmad Raus, Raha
Ali, Abdul Manaf
Tan, Wen Siang
Mohd. Salleh, Hamzah
Eshagi, Majid
Yusoff, Khatijah
author_sort Ahmad Raus, Raha
collection IIUM
description A panel of six monoclonal antibodies against the nucleocapsid protein (NP) of Newcastle disease virus (NDV) strain AF2240 was produced by immunization of Balb/c mice with purified NP. Isotyping test revealed three of the monoclonal (mAbs) were IgG1 and the others were IgG2a. All of the mAbs recognized linearized NP epitopes. The reactivity of these mAbs with local isolates revealed that three mAbs recognized conserved antigenic sites and the others bind to antigenic sites which undergone considerable changes.
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spelling oai:generic.eprints.org:292782021-06-18T02:43:33Z http://irep.iium.edu.my/29278/ Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus Ahmad Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Eshagi, Majid Yusoff, Khatijah Q Science (General) QR355 Virology SF Animal culture A panel of six monoclonal antibodies against the nucleocapsid protein (NP) of Newcastle disease virus (NDV) strain AF2240 was produced by immunization of Balb/c mice with purified NP. Isotyping test revealed three of the monoclonal (mAbs) were IgG1 and the others were IgG2a. All of the mAbs recognized linearized NP epitopes. The reactivity of these mAbs with local isolates revealed that three mAbs recognized conserved antigenic sites and the others bind to antigenic sites which undergone considerable changes. 2007 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/29278/1/ICBioE_Paper_raha1%27_2007.pdf Ahmad Raus, Raha and Ali, Abdul Manaf and Tan, Wen Siang and Mohd. Salleh, Hamzah and Eshagi, Majid and Yusoff, Khatijah (2007) Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus. In: International Conference on Biotechnology Engineering (ICBioE’07), 8 - 10 May 2007, Kuala Lumpur.
spellingShingle Q Science (General)
QR355 Virology
SF Animal culture
Ahmad Raus, Raha
Ali, Abdul Manaf
Tan, Wen Siang
Mohd. Salleh, Hamzah
Eshagi, Majid
Yusoff, Khatijah
Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus
title Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus
title_full Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus
title_fullStr Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus
title_full_unstemmed Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus
title_short Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus
title_sort production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant newcastle disease virus
topic Q Science (General)
QR355 Virology
SF Animal culture
url http://irep.iium.edu.my/29278/1/ICBioE_Paper_raha1%27_2007.pdf
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