Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus

Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli. One of the antigenic sites was located within amino acids 441 to 489 of C-terminal. Two other antigenic sites located within the Nterminal of NP protein from amino acids 26 to 121...

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Main Authors: Ahmad Raus, Raha, Ali, Abdul Manaf, Tan, Wen Siang, Mohd. Salleh, Hamzah, Eshagi, Majid, Yusoff, Khatijah
Format: Proceeding Paper
Language:English
Published: 2007
Subjects:
Online Access:http://irep.iium.edu.my/29279/1/ICBioE_Paper_raha2%27_2007.pdf
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author Ahmad Raus, Raha
Ali, Abdul Manaf
Tan, Wen Siang
Mohd. Salleh, Hamzah
Eshagi, Majid
Yusoff, Khatijah
author_facet Ahmad Raus, Raha
Ali, Abdul Manaf
Tan, Wen Siang
Mohd. Salleh, Hamzah
Eshagi, Majid
Yusoff, Khatijah
author_sort Ahmad Raus, Raha
collection IIUM
description Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli. One of the antigenic sites was located within amino acids 441 to 489 of C-terminal. Two other antigenic sites located within the Nterminal of NP protein from amino acids 26 to 121 and 122 to 375 residues. Identification of NP antigenic sites not only elucidates the NP sequences that are responsible in eliciting immune response, indirectly it also revealed which sequences are exposed on the NP herringbone-like structure.
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spelling oai:generic.eprints.org:292792021-06-18T04:51:27Z http://irep.iium.edu.my/29279/ Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus Ahmad Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Eshagi, Majid Yusoff, Khatijah Q Science (General) QR355 Virology Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli. One of the antigenic sites was located within amino acids 441 to 489 of C-terminal. Two other antigenic sites located within the Nterminal of NP protein from amino acids 26 to 121 and 122 to 375 residues. Identification of NP antigenic sites not only elucidates the NP sequences that are responsible in eliciting immune response, indirectly it also revealed which sequences are exposed on the NP herringbone-like structure. 2007 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/29279/1/ICBioE_Paper_raha2%27_2007.pdf Ahmad Raus, Raha and Ali, Abdul Manaf and Tan, Wen Siang and Mohd. Salleh, Hamzah and Eshagi, Majid and Yusoff, Khatijah (2007) Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus. In: International Conference on Biotechnology Engineering 2007, 8 - 10 May 2007, Kuala Lumpur.
spellingShingle Q Science (General)
QR355 Virology
Ahmad Raus, Raha
Ali, Abdul Manaf
Tan, Wen Siang
Mohd. Salleh, Hamzah
Eshagi, Majid
Yusoff, Khatijah
Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus
title Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus
title_full Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus
title_fullStr Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus
title_full_unstemmed Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus
title_short Localization of nucleocapsid (NP) antigenic sites by using a panel of monoclonal antibodies against the recombinant NP of Newcastle disease virus
title_sort localization of nucleocapsid np antigenic sites by using a panel of monoclonal antibodies against the recombinant np of newcastle disease virus
topic Q Science (General)
QR355 Virology
url http://irep.iium.edu.my/29279/1/ICBioE_Paper_raha2%27_2007.pdf
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