Improvement of filtration process for nucleocapsid protein of Newcastle disease virus
This research was mainly focusing on improving the purification process for recombinant nucleocapsid (NP) protein of Newcastle disease virus (NDV). In this study, several conditions of the independent variables that affect the microfiltration process such as the temperature, transmembrane pressur...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/29280/4/Improvement_of_Filtration_Process.pdf |
| _version_ | 1825647185074585600 |
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| author | Ahmad Raus, Raha Awang, Rabiatul Adawiyah Mel, Maizirwan Yusoff, Khatijah |
| author_facet | Ahmad Raus, Raha Awang, Rabiatul Adawiyah Mel, Maizirwan Yusoff, Khatijah |
| author_sort | Ahmad Raus, Raha |
| collection | IIUM |
| description | This research was mainly focusing on improving the purification process for
recombinant nucleocapsid (NP) protein of Newcastle disease virus (NDV). In this
study, several conditions of the independent variables that affect the microfiltration
process such as the temperature, transmembrane pressure (TMP), and viscosity were
observed. A microporous membrane screening using two different sizes of membrane
which are 0.1μm and 0.45μm was performed before further improvement on NP
protein filtration was carried out. It turned out to be that 0.45μm membrane gave
higher yield of NP protein than that of 0.1μm membrane. Thus, 0.45μm membrane
was used to improve the filtration process for NP protein. Based on the Full Factorial
Design (STATISTICA 8.0, Statsoft, Inc.), eight sets of experiment were designed to
identify the best conditions for the NP protein filtration. From these experiments, the
optimal conditions that gave the highest yield of NP protein are; TMP 4.5psi,
viscosity 2.39cP at 4ºC. Based on ANOVA (analysis of variance), all the independent
variables studied do not significantly affect the purification of NP protein. However,
based on the lowest p value for each variable corresponding to each response,
temperature has the largest effect for the NP protein yield. |
| first_indexed | 2024-03-05T23:13:51Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:29280 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:13:51Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:292802013-06-25T11:17:39Z http://irep.iium.edu.my/29280/ Improvement of filtration process for nucleocapsid protein of Newcastle disease virus Ahmad Raus, Raha Awang, Rabiatul Adawiyah Mel, Maizirwan Yusoff, Khatijah Q Science (General) QR355 Virology This research was mainly focusing on improving the purification process for recombinant nucleocapsid (NP) protein of Newcastle disease virus (NDV). In this study, several conditions of the independent variables that affect the microfiltration process such as the temperature, transmembrane pressure (TMP), and viscosity were observed. A microporous membrane screening using two different sizes of membrane which are 0.1μm and 0.45μm was performed before further improvement on NP protein filtration was carried out. It turned out to be that 0.45μm membrane gave higher yield of NP protein than that of 0.1μm membrane. Thus, 0.45μm membrane was used to improve the filtration process for NP protein. Based on the Full Factorial Design (STATISTICA 8.0, Statsoft, Inc.), eight sets of experiment were designed to identify the best conditions for the NP protein filtration. From these experiments, the optimal conditions that gave the highest yield of NP protein are; TMP 4.5psi, viscosity 2.39cP at 4ºC. Based on ANOVA (analysis of variance), all the independent variables studied do not significantly affect the purification of NP protein. However, based on the lowest p value for each variable corresponding to each response, temperature has the largest effect for the NP protein yield. 2009 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/29280/4/Improvement_of_Filtration_Process.pdf Ahmad Raus, Raha and Awang, Rabiatul Adawiyah and Mel, Maizirwan and Yusoff, Khatijah (2009) Improvement of filtration process for nucleocapsid protein of Newcastle disease virus. In: Malaysian International Conference on Trends in Bioprocess Engineering., 12 - 13 December 2009, Kuala Lumpur. |
| spellingShingle | Q Science (General) QR355 Virology Ahmad Raus, Raha Awang, Rabiatul Adawiyah Mel, Maizirwan Yusoff, Khatijah Improvement of filtration process for nucleocapsid protein of Newcastle disease virus |
| title | Improvement of filtration process for nucleocapsid protein of Newcastle disease virus |
| title_full | Improvement of filtration process for nucleocapsid protein of Newcastle disease virus |
| title_fullStr | Improvement of filtration process for nucleocapsid protein of Newcastle disease virus |
| title_full_unstemmed | Improvement of filtration process for nucleocapsid protein of Newcastle disease virus |
| title_short | Improvement of filtration process for nucleocapsid protein of Newcastle disease virus |
| title_sort | improvement of filtration process for nucleocapsid protein of newcastle disease virus |
| topic | Q Science (General) QR355 Virology |
| url | http://irep.iium.edu.my/29280/4/Improvement_of_Filtration_Process.pdf |
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