Comparison of growth rate and viability of DF-1 cell line in different culture media
One of the commercial cell line that gained attention for the study of viral vaccine was an immortalized cell line derived from chicken embryo fibroblast (CEF) cells. Unlike CEF cells, DF-1 cells have higher proliferation rate and also can be subcultured infinite number of times. In this study, th...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2008
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/29284/1/zPaper_no_14_for_ICSTIE__Mohd_Azmir-edited_.pdf |
| _version_ | 1825647185710022656 |
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| author | Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini |
| author_facet | Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini |
| author_sort | Arifin, Mohd Azmir |
| collection | IIUM |
| description | One of the commercial cell line that gained attention for the study of viral vaccine was an immortalized cell
line derived from chicken embryo fibroblast (CEF) cells. Unlike CEF cells, DF-1 cells have higher proliferation rate
and also can be subcultured infinite number of times. In this study, the growth profile of DF-1 cell line was investigated
by undergoing series of optimization process by testing it out on several culture media and also by manipulating the
components of culture media which were sodium bicarbonate and serum. At the end of this study it has been figured out
that DF-1 achieved the highest cell culture density in Dulbecco’s Modification of Eagle’s Medium( DMEM) and with
1.24 g/L of NaHCO3 and 5.5 % serum, it yielded the shortest doubling time. |
| first_indexed | 2024-03-05T23:13:52Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:29284 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-13T19:10:05Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:292842013-04-24T08:10:05Z http://irep.iium.edu.my/29284/ Comparison of growth rate and viability of DF-1 cell line in different culture media Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini Q Science (General) SF Animal culture One of the commercial cell line that gained attention for the study of viral vaccine was an immortalized cell line derived from chicken embryo fibroblast (CEF) cells. Unlike CEF cells, DF-1 cells have higher proliferation rate and also can be subcultured infinite number of times. In this study, the growth profile of DF-1 cell line was investigated by undergoing series of optimization process by testing it out on several culture media and also by manipulating the components of culture media which were sodium bicarbonate and serum. At the end of this study it has been figured out that DF-1 achieved the highest cell culture density in Dulbecco’s Modification of Eagle’s Medium( DMEM) and with 1.24 g/L of NaHCO3 and 5.5 % serum, it yielded the shortest doubling time. 2008 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/29284/1/zPaper_no_14_for_ICSTIE__Mohd_Azmir-edited_.pdf Arifin, Mohd Azmir and Mel, Maizirwan and Ahmad Raus, Raha and S. H., Sharifah and Ideris, Aini (2008) Comparison of growth rate and viability of DF-1 cell line in different culture media. In: 2nd International Conference on Science and Technology. , 12 - 13 December 2008, Pulau Pinang. |
| spellingShingle | Q Science (General) SF Animal culture Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini Comparison of growth rate and viability of DF-1 cell line in different culture media |
| title | Comparison of growth rate and viability of DF-1 cell line in different culture media |
| title_full | Comparison of growth rate and viability of DF-1 cell line in different culture media |
| title_fullStr | Comparison of growth rate and viability of DF-1 cell line in different culture media |
| title_full_unstemmed | Comparison of growth rate and viability of DF-1 cell line in different culture media |
| title_short | Comparison of growth rate and viability of DF-1 cell line in different culture media |
| title_sort | comparison of growth rate and viability of df 1 cell line in different culture media |
| topic | Q Science (General) SF Animal culture |
| url | http://irep.iium.edu.my/29284/1/zPaper_no_14_for_ICSTIE__Mohd_Azmir-edited_.pdf |
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