Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice
Plants leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected, freeze dried and extracted with aqueous methanol. The b...
| Main Authors: | , , , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/29733/1/Muhammad_Muzaffar_Ali_Khan_Khattak-1.pdf http://irep.iium.edu.my/29733/2/MSTC_SCHEDULE.pdf |
| _version_ | 1796877967057485824 |
|---|---|
| author | Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Abdul Rahman, Suzanah Damanik , Rizal Yahaya, Azhary |
| author_facet | Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Abdul Rahman, Suzanah Damanik , Rizal Yahaya, Azhary |
| author_sort | Khattak, Muhammad Muzaffar Ali Khan |
| collection | IIUM |
| description | Plants leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected, freeze dried and extracted with aqueous methanol. The biological activity in vitro, in relation to total phenolic, flavonoid & antioxidants contents and antimicrobial & antifungal activities were assessed. Total phenolics & antioxidants activities, antimicrobial & anti-fungal activities and toxicity were determined by using appropriate methods. The phenolic content among the extracts of leaves showed significant (p<0.05) difference ranging from 55.21 - 95.17 mg GAE/g of dried samples. The PC-M had the highest contents of phenolics followed by CBP-M. There were significant (p<0.05) differences in IC50 values ranging from 10.5 – 34.1 µg/ml. Among the species studied, CAL-M and PC-M showed higher antioxidants activities compared to the rest of the extracts. The extracts showed activities against bacteria and fungus and resulted in significant (p<0.05) differences between activities against microorganisms and fungus. The minimum inhibitory concentration of all leaves extracts ranged from 1.0–2.0 mg/ml in inhibiting the growth of S. aureus, E. coli, P. aeruginosa and B.subtilis. The acute toxicity test revealed that there was no mortality even at the highest doses levels used in mice i.e. 5000 mg/kg body weight. This study shows that the extracts can be used as antioxidants and antimicrobial agent without having any toxic effect. Furthermore, the anti- antimicrobial & anti-fungal activities relates to the concentration of total phenolic, flavonoid and antioxidants in the extracts. |
| first_indexed | 2024-03-05T23:14:50Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:29733 |
| institution | International Islamic University Malaysia |
| language | English English |
| last_indexed | 2024-03-05T23:14:50Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:297332014-12-09T01:04:26Z http://irep.iium.edu.my/29733/ Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Abdul Rahman, Suzanah Damanik , Rizal Yahaya, Azhary TX341 Nutrition. Foods and food supply Plants leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected, freeze dried and extracted with aqueous methanol. The biological activity in vitro, in relation to total phenolic, flavonoid & antioxidants contents and antimicrobial & antifungal activities were assessed. Total phenolics & antioxidants activities, antimicrobial & anti-fungal activities and toxicity were determined by using appropriate methods. The phenolic content among the extracts of leaves showed significant (p<0.05) difference ranging from 55.21 - 95.17 mg GAE/g of dried samples. The PC-M had the highest contents of phenolics followed by CBP-M. There were significant (p<0.05) differences in IC50 values ranging from 10.5 – 34.1 µg/ml. Among the species studied, CAL-M and PC-M showed higher antioxidants activities compared to the rest of the extracts. The extracts showed activities against bacteria and fungus and resulted in significant (p<0.05) differences between activities against microorganisms and fungus. The minimum inhibitory concentration of all leaves extracts ranged from 1.0–2.0 mg/ml in inhibiting the growth of S. aureus, E. coli, P. aeruginosa and B.subtilis. The acute toxicity test revealed that there was no mortality even at the highest doses levels used in mice i.e. 5000 mg/kg body weight. This study shows that the extracts can be used as antioxidants and antimicrobial agent without having any toxic effect. Furthermore, the anti- antimicrobial & anti-fungal activities relates to the concentration of total phenolic, flavonoid and antioxidants in the extracts. 2012 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/29733/1/Muhammad_Muzaffar_Ali_Khan_Khattak-1.pdf application/pdf en http://irep.iium.edu.my/29733/2/MSTC_SCHEDULE.pdf Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher and Abdul Rahman, Suzanah and Damanik , Rizal and Yahaya, Azhary (2012) Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice. In: Malaysian Sciences and Technology Congress 2012 , 20 November 2012. (Unpublished) |
| spellingShingle | TX341 Nutrition. Foods and food supply Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Abdul Rahman, Suzanah Damanik , Rizal Yahaya, Azhary Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice |
| title | Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice |
| title_full | Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice |
| title_fullStr | Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice |
| title_full_unstemmed | Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice |
| title_short | Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice |
| title_sort | coleus amboinicus exhibits anti microbial and anti fungus activities and having no toxicity at highest doses assessed in mice |
| topic | TX341 Nutrition. Foods and food supply |
| url | http://irep.iium.edu.my/29733/1/Muhammad_Muzaffar_Ali_Khan_Khattak-1.pdf http://irep.iium.edu.my/29733/2/MSTC_SCHEDULE.pdf |
| work_keys_str_mv | AT khattakmuhammadmuzaffaralikhan coleusamboinicusexhibitsantimicrobialandantifungusactivitiesandhavingnotoxicityathighestdosesassessedinmice AT bakhtiarmtaher coleusamboinicusexhibitsantimicrobialandantifungusactivitiesandhavingnotoxicityathighestdosesassessedinmice AT abdulrahmansuzanah coleusamboinicusexhibitsantimicrobialandantifungusactivitiesandhavingnotoxicityathighestdosesassessedinmice AT damanikrizal coleusamboinicusexhibitsantimicrobialandantifungusactivitiesandhavingnotoxicityathighestdosesassessedinmice AT yahayaazhary coleusamboinicusexhibitsantimicrobialandantifungusactivitiesandhavingnotoxicityathighestdosesassessedinmice |