Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1
Coolia is a widespread and ecologically important genus of benthic marine dinoflagellates found in tropical regions. Historically, there has been taxonomic confusion about the taxonomy and toxicity of this group. The goal of this study was to resolve morphological questions concerning Coolia t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Blackwell Publishing
2013
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/30406/1/Coolia_tropicalis.pdf |
| _version_ | 1796878042044301312 |
|---|---|
| author | Mohammad Noor, Normawaty Moestrup, Ojvind Lundholm, Nina Fraga, Santiago Adam, Aimimuliani Holmes, Michael Saleh, Ejria |
| author_facet | Mohammad Noor, Normawaty Moestrup, Ojvind Lundholm, Nina Fraga, Santiago Adam, Aimimuliani Holmes, Michael Saleh, Ejria |
| author_sort | Mohammad Noor, Normawaty |
| collection | IIUM |
| description | Coolia is a widespread and ecologically important
genus of benthic marine dinoflagellates found in
tropical regions. Historically, there has been
taxonomic confusion about the taxonomy and
toxicity of this group. The goal of this study was to
resolve morphological questions concerning Coolia
tropicalis and determine the taxonomic identity of the
Australian Coolia isolate which has been reported to
produce cooliatoxins. To accomplish this, the
morphology of tropical strains from Belize (the type
locality of C. tropicalis), Malaysia, Indonesia, and
Australia were examined and compared to published
reports. The morphological analysis showed that
C. tropicalis differs from the original description in
that it has a slightly larger size (35–47 lm long by
30–45 lm wide versus 23–40 lm long by 25–39 lm
wide), and the shape of fourth apical plate, and the
length of Po plate (7.4–12 lm versus 7 lm). Based
on both morphology and phylogenetic analysis using
LSU D1- D3 rDNA sequences, the clones of
C. tropicalis from Malaysia, Indonesia, and Belize
were found to form a monophyletic clade within the
genus. The strain producing cooliatoxin was found to
be C. tropicalis, not Coolia monotis as originally
assumed. To explore the factors influencing the
growth of Coolia species, the growth rates of
C. tropicalis and Coolia malayensis were determined at
different temperatures and salinities. Both species
tolerated a wide range of temperatures, but cannot
survive at temperatures <20°C or >35°C. C. monotis,
the dominant species reported in the literature,
probably does not produce toxins. |
| first_indexed | 2024-03-05T23:15:57Z |
| format | Article |
| id | oai:generic.eprints.org:30406 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:15:57Z |
| publishDate | 2013 |
| publisher | Blackwell Publishing |
| record_format | dspace |
| spelling | oai:generic.eprints.org:304062013-08-26T22:59:06Z http://irep.iium.edu.my/30406/ Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1 Mohammad Noor, Normawaty Moestrup, Ojvind Lundholm, Nina Fraga, Santiago Adam, Aimimuliani Holmes, Michael Saleh, Ejria Q Science (General) Coolia is a widespread and ecologically important genus of benthic marine dinoflagellates found in tropical regions. Historically, there has been taxonomic confusion about the taxonomy and toxicity of this group. The goal of this study was to resolve morphological questions concerning Coolia tropicalis and determine the taxonomic identity of the Australian Coolia isolate which has been reported to produce cooliatoxins. To accomplish this, the morphology of tropical strains from Belize (the type locality of C. tropicalis), Malaysia, Indonesia, and Australia were examined and compared to published reports. The morphological analysis showed that C. tropicalis differs from the original description in that it has a slightly larger size (35–47 lm long by 30–45 lm wide versus 23–40 lm long by 25–39 lm wide), and the shape of fourth apical plate, and the length of Po plate (7.4–12 lm versus 7 lm). Based on both morphology and phylogenetic analysis using LSU D1- D3 rDNA sequences, the clones of C. tropicalis from Malaysia, Indonesia, and Belize were found to form a monophyletic clade within the genus. The strain producing cooliatoxin was found to be C. tropicalis, not Coolia monotis as originally assumed. To explore the factors influencing the growth of Coolia species, the growth rates of C. tropicalis and Coolia malayensis were determined at different temperatures and salinities. Both species tolerated a wide range of temperatures, but cannot survive at temperatures <20°C or >35°C. C. monotis, the dominant species reported in the literature, probably does not produce toxins. Blackwell Publishing 2013-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30406/1/Coolia_tropicalis.pdf Mohammad Noor, Normawaty and Moestrup, Ojvind and Lundholm, Nina and Fraga, Santiago and Adam, Aimimuliani and Holmes, Michael and Saleh, Ejria (2013) Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1. Journal of Phycology, 49 (3). pp. 536-545. ISSN 0022-3646 http://onlinelibrary.wiley.com/doi/10.1111/jpy.12062/abstract 10.1111/jpy.12062 |
| spellingShingle | Q Science (General) Mohammad Noor, Normawaty Moestrup, Ojvind Lundholm, Nina Fraga, Santiago Adam, Aimimuliani Holmes, Michael Saleh, Ejria Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1 |
| title | Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1 |
| title_full | Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1 |
| title_fullStr | Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1 |
| title_full_unstemmed | Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1 |
| title_short | Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA1 |
| title_sort | autecology and phylogeny of coolia tropicalis and coolia malayensis dinophyceae with emphasis on taxonomy of c tropicalis based on light microscopy scanning electron microscopy and lsu rdna1 |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/30406/1/Coolia_tropicalis.pdf |
| work_keys_str_mv | AT mohammadnoornormawaty autecologyandphylogenyofcooliatropicalisandcooliamalayensisdinophyceaewithemphasisontaxonomyofctropicalisbasedonlightmicroscopyscanningelectronmicroscopyandlsurdna1 AT moestrupojvind autecologyandphylogenyofcooliatropicalisandcooliamalayensisdinophyceaewithemphasisontaxonomyofctropicalisbasedonlightmicroscopyscanningelectronmicroscopyandlsurdna1 AT lundholmnina autecologyandphylogenyofcooliatropicalisandcooliamalayensisdinophyceaewithemphasisontaxonomyofctropicalisbasedonlightmicroscopyscanningelectronmicroscopyandlsurdna1 AT fragasantiago autecologyandphylogenyofcooliatropicalisandcooliamalayensisdinophyceaewithemphasisontaxonomyofctropicalisbasedonlightmicroscopyscanningelectronmicroscopyandlsurdna1 AT adamaimimuliani autecologyandphylogenyofcooliatropicalisandcooliamalayensisdinophyceaewithemphasisontaxonomyofctropicalisbasedonlightmicroscopyscanningelectronmicroscopyandlsurdna1 AT holmesmichael autecologyandphylogenyofcooliatropicalisandcooliamalayensisdinophyceaewithemphasisontaxonomyofctropicalisbasedonlightmicroscopyscanningelectronmicroscopyandlsurdna1 AT salehejria autecologyandphylogenyofcooliatropicalisandcooliamalayensisdinophyceaewithemphasisontaxonomyofctropicalisbasedonlightmicroscopyscanningelectronmicroscopyandlsurdna1 |