| Summary: | The antioxidant activity and the total phenolic content, as well as the influence of petroleum ether, chloroform and methanol extracts from the leaves of Gynotroches axillaris, on micro- organisms were studied. The total phenolic contents were evaluated by using Folin-Ciocalteu reagent and the obtained values ranged from 70.0 to 620 mg GAE/g. The efficiency of antiox- idation, which was identified through the scavenging of free radical DPPH, exhibited that the highest IC50 was in the methanolic extract (44.7 μg/mL) as compared to the standard ascorbic acid (25.83 μg/mL) and to standard BHT (17.2 μg/mL). In vitro antimicrobial activity of ex- tracts was tested against Gram-negative bacteria, Gram-positive bacteria and fungi. Methanol extract showed activity in the range (225-900 μg/mL) with both types, while petroleum ether and chloroform extracts were only active with Bacillus subtilis. The three extracts strongly inhibited all fungi with activity 225-450 μg/mL. The toxicity test against brine shrimps indi- cated that all extracts were non-toxic with LC50 value more than 1000 μg/mL. The finding of this study supports the safety of these extracts to be used in medical treatments.
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