Bioremediation of hydrocarbon-contamidated Soils

Crude oil spills resulting from the leakage from underground storage tanks, pipelines and other industrial operations are an important source of soil contamination. Bioremediation is an emerging technology that is used to recover contaminated soils. Laboratory scale experiments were carried out to b...

Full description

Bibliographic Details
Main Authors: Muyibi, Suleyman Aremu, Alkhatib, Ma'an Fahmi Rashid, Alam, Md. Zahangir, Kabbashi, Nassereldeen Ahmed
Format: Monograph
Language:English
Published: [s.n] 2012
Subjects:
Online Access:http://irep.iium.edu.my/31229/1/EDW_B_0903-237.pdf
_version_ 1796878131104055296
author Muyibi, Suleyman Aremu
Alkhatib, Ma'an Fahmi Rashid
Alam, Md. Zahangir
Kabbashi, Nassereldeen Ahmed
author_facet Muyibi, Suleyman Aremu
Alkhatib, Ma'an Fahmi Rashid
Alam, Md. Zahangir
Kabbashi, Nassereldeen Ahmed
author_sort Muyibi, Suleyman Aremu
collection IIUM
description Crude oil spills resulting from the leakage from underground storage tanks, pipelines and other industrial operations are an important source of soil contamination. Bioremediation is an emerging technology that is used to recover contaminated soils. Laboratory scale experiments were carried out to bioremediate crude oil contaminated soil (COCS) using isolated bacterial consortium and domestic waste water sludge as nutrient source. The bacterial strains were isolated from COCS by enrichment culture using minimal salt medium (MSM) with crude oil as sole carbon source. The isolated strains were purified and monitored for their growth by monitoring the optical density (OD600). Four bacterial consortiums were formed and monitored for their growth on MSM with crude oil as sole carbon source. The fastest growing consortium (IMN201) was selected for the kinetics study and the biodegradability test in soil slurry. The bioremediation experiments were designed using DesignExpert 6.0.8 software by optimizing the amount of crude oil, microbial inoculum and sludge which are initially added to the autoclaved soil. Fifteen runs were carried out until no more microbial activity was noticed. The biodegradability test showed 95.8% removal of TPH within 7 days of incubation while the kinetics parameters were µmax = 0.02 hr1 and ks = 2.2% v/v oil. From the bioremediation results, the optimum degradation obtained was 99.3% within 13 days.
first_indexed 2024-03-05T23:17:09Z
format Monograph
id oai:generic.eprints.org:31229
institution International Islamic University Malaysia
language English
last_indexed 2024-03-05T23:17:09Z
publishDate 2012
publisher [s.n]
record_format dspace
spelling oai:generic.eprints.org:312292013-09-30T09:00:42Z http://irep.iium.edu.my/31229/ Bioremediation of hydrocarbon-contamidated Soils Muyibi, Suleyman Aremu Alkhatib, Ma'an Fahmi Rashid Alam, Md. Zahangir Kabbashi, Nassereldeen Ahmed TA Engineering (General). Civil engineering (General) Crude oil spills resulting from the leakage from underground storage tanks, pipelines and other industrial operations are an important source of soil contamination. Bioremediation is an emerging technology that is used to recover contaminated soils. Laboratory scale experiments were carried out to bioremediate crude oil contaminated soil (COCS) using isolated bacterial consortium and domestic waste water sludge as nutrient source. The bacterial strains were isolated from COCS by enrichment culture using minimal salt medium (MSM) with crude oil as sole carbon source. The isolated strains were purified and monitored for their growth by monitoring the optical density (OD600). Four bacterial consortiums were formed and monitored for their growth on MSM with crude oil as sole carbon source. The fastest growing consortium (IMN201) was selected for the kinetics study and the biodegradability test in soil slurry. The bioremediation experiments were designed using DesignExpert 6.0.8 software by optimizing the amount of crude oil, microbial inoculum and sludge which are initially added to the autoclaved soil. Fifteen runs were carried out until no more microbial activity was noticed. The biodegradability test showed 95.8% removal of TPH within 7 days of incubation while the kinetics parameters were µmax = 0.02 hr1 and ks = 2.2% v/v oil. From the bioremediation results, the optimum degradation obtained was 99.3% within 13 days. [s.n] 2012-05-07 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/31229/1/EDW_B_0903-237.pdf Muyibi, Suleyman Aremu and Alkhatib, Ma'an Fahmi Rashid and Alam, Md. Zahangir and Kabbashi, Nassereldeen Ahmed (2012) Bioremediation of hydrocarbon-contamidated Soils. Research Report. [s.n]. (Unpublished)
spellingShingle TA Engineering (General). Civil engineering (General)
Muyibi, Suleyman Aremu
Alkhatib, Ma'an Fahmi Rashid
Alam, Md. Zahangir
Kabbashi, Nassereldeen Ahmed
Bioremediation of hydrocarbon-contamidated Soils
title Bioremediation of hydrocarbon-contamidated Soils
title_full Bioremediation of hydrocarbon-contamidated Soils
title_fullStr Bioremediation of hydrocarbon-contamidated Soils
title_full_unstemmed Bioremediation of hydrocarbon-contamidated Soils
title_short Bioremediation of hydrocarbon-contamidated Soils
title_sort bioremediation of hydrocarbon contamidated soils
topic TA Engineering (General). Civil engineering (General)
url http://irep.iium.edu.my/31229/1/EDW_B_0903-237.pdf
work_keys_str_mv AT muyibisuleymanaremu bioremediationofhydrocarboncontamidatedsoils
AT alkhatibmaanfahmirashid bioremediationofhydrocarboncontamidatedsoils
AT alammdzahangir bioremediationofhydrocarboncontamidatedsoils
AT kabbashinassereldeenahmed bioremediationofhydrocarboncontamidatedsoils