LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study
A simple, precise and reproducible liquid chromatography–tandem mass spectrometry method has been developed and validated according to the Food and Drug Administration guidelines for the simultaneous quantitation of antidiabetic drugs metformin, glimiperide and pioglitazone in human plasma using gli...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Vieweg Verlag
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/31908/1/Paper6.pdf |
| _version_ | 1825647457112948736 |
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| author | Sengupta, Pinaki Bhaumik, Uttam Ghosh, Animesh Sarkar, Amlan Kanti Chatterjee, Bappaditya Bose, Anirbandeep Pal, Tapan Kumar |
| author_facet | Sengupta, Pinaki Bhaumik, Uttam Ghosh, Animesh Sarkar, Amlan Kanti Chatterjee, Bappaditya Bose, Anirbandeep Pal, Tapan Kumar |
| author_sort | Sengupta, Pinaki |
| collection | IIUM |
| description | A simple, precise and reproducible liquid chromatography–tandem mass spectrometry method has been developed and validated according to the Food and Drug Administration guidelines for the simultaneous quantitation of antidiabetic drugs metformin, glimiperide and pioglitazone in human plasma using glipizide as an internal standard. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. Inter-batch and intra-batch coefficient of variation across four validation runs for the quality control samples was less than 7%. The accuracy determined at quality control levels was within 92.81–105.13%. The method was applied to a bioequivalence study. |
| first_indexed | 2024-03-05T23:17:53Z |
| format | Article |
| id | oai:generic.eprints.org:31908 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:17:53Z |
| publishDate | 2009 |
| publisher | Vieweg Verlag |
| record_format | dspace |
| spelling | oai:generic.eprints.org:319082013-09-20T03:10:46Z http://irep.iium.edu.my/31908/ LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study Sengupta, Pinaki Bhaumik, Uttam Ghosh, Animesh Sarkar, Amlan Kanti Chatterjee, Bappaditya Bose, Anirbandeep Pal, Tapan Kumar R Medicine (General) A simple, precise and reproducible liquid chromatography–tandem mass spectrometry method has been developed and validated according to the Food and Drug Administration guidelines for the simultaneous quantitation of antidiabetic drugs metformin, glimiperide and pioglitazone in human plasma using glipizide as an internal standard. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. Inter-batch and intra-batch coefficient of variation across four validation runs for the quality control samples was less than 7%. The accuracy determined at quality control levels was within 92.81–105.13%. The method was applied to a bioequivalence study. Vieweg Verlag 2009-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/31908/1/Paper6.pdf Sengupta, Pinaki and Bhaumik, Uttam and Ghosh, Animesh and Sarkar, Amlan Kanti and Chatterjee, Bappaditya and Bose, Anirbandeep and Pal, Tapan Kumar (2009) LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study. Chromatographia, 69 (11/12). pp. 1243-1250. ISSN 1612-1112 (O), 0009-5893 (P) http://link.springer.com/article/10.1365%2Fs10337-009-1056-5 10.1365/s10337-009-1056-5 |
| spellingShingle | R Medicine (General) Sengupta, Pinaki Bhaumik, Uttam Ghosh, Animesh Sarkar, Amlan Kanti Chatterjee, Bappaditya Bose, Anirbandeep Pal, Tapan Kumar LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study |
| title | LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study |
| title_full | LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study |
| title_fullStr | LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study |
| title_full_unstemmed | LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study |
| title_short | LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study |
| title_sort | lc ms ms development and validation for simultaneous quantitation of metformin glimepiride and pioglitazone in human plasma and its application to a bioequivalence study |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/31908/1/Paper6.pdf |
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