Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system
The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol f...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2013
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| Online Access: | http://irep.iium.edu.my/32047/1/rahmani_JLCRT_2013.pdf |
| _version_ | 1796878202802536448 |
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| author | Rahmani, Anoshep Selamat, Jinap Khatib, Alfi Chin Ping, Tan |
| author_facet | Rahmani, Anoshep Selamat, Jinap Khatib, Alfi Chin Ping, Tan |
| author_sort | Rahmani, Anoshep |
| collection | IIUM |
| description | The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol followed by a multifunctional immunoaffinity AOZ column clean-up was applied for the extraction of these mycotoxins before HPLC analysis. A photochemical reactor enhance derivatization system (PHRED) was used between the HPLC column and fluorescence
detector, in order to enhance the fluorescence intensity of aflatoxin B1 and G1. The best chromatographic separation for the simultaneous determination of the nominated mycotoxins was achieved under optimal HPLC conditions. Recoveries were 77.31–104.1% for cereal samples spiked at 6.5,10, and 100 ng=g for total aflatoxins (AFs), OTA, and ZEA, respectively; whereas, the precisions were 2.45–11.14%. Limits of detection (LOD) for AFs, OTA, and ZEA were 0.004–0.012,0.05,
and 0.5 ng=g, respectively; whereas. limits of quantification (LOQ) were 0.015–0.05, 0.2, and 2ng=g, correspondingly. |
| first_indexed | 2024-03-05T23:18:08Z |
| format | Article |
| id | oai:generic.eprints.org:32047 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:18:08Z |
| publishDate | 2013 |
| publisher | Taylor & Francis Group |
| record_format | dspace |
| spelling | oai:generic.eprints.org:320472013-09-30T02:53:13Z http://irep.iium.edu.my/32047/ Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system Rahmani, Anoshep Selamat, Jinap Khatib, Alfi Chin Ping, Tan QD Chemistry The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol followed by a multifunctional immunoaffinity AOZ column clean-up was applied for the extraction of these mycotoxins before HPLC analysis. A photochemical reactor enhance derivatization system (PHRED) was used between the HPLC column and fluorescence detector, in order to enhance the fluorescence intensity of aflatoxin B1 and G1. The best chromatographic separation for the simultaneous determination of the nominated mycotoxins was achieved under optimal HPLC conditions. Recoveries were 77.31–104.1% for cereal samples spiked at 6.5,10, and 100 ng=g for total aflatoxins (AFs), OTA, and ZEA, respectively; whereas, the precisions were 2.45–11.14%. Limits of detection (LOD) for AFs, OTA, and ZEA were 0.004–0.012,0.05, and 0.5 ng=g, respectively; whereas. limits of quantification (LOQ) were 0.015–0.05, 0.2, and 2ng=g, correspondingly. Taylor & Francis Group 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32047/1/rahmani_JLCRT_2013.pdf Rahmani, Anoshep and Selamat, Jinap and Khatib, Alfi and Chin Ping, Tan (2013) Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system. Journal of Liquid Chromatography & Related Technologies, 36 (5). pp. 600-617. ISSN 1082-6076 print/1520-572X online http://www.tandfonline.com/doi/full/10.1080/10826076.2012.670182#.UkjtQn_RI9k 0.1080/10826076.2012.670182 |
| spellingShingle | QD Chemistry Rahmani, Anoshep Selamat, Jinap Khatib, Alfi Chin Ping, Tan Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system |
| title | Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system |
| title_full | Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system |
| title_fullStr | Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system |
| title_full_unstemmed | Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system |
| title_short | Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system |
| title_sort | simultaneous determination of aflatoxins ochratoxin a and zearalenone in cereals using a validated hplc fld method and phred derivatization system |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/32047/1/rahmani_JLCRT_2013.pdf |
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