Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone
The potential of thymoquinone, an active component of Nigella Sativa, in lowering chemotherapeuticassociated toxicity of cyclophosphamide in the testis, epididymis and sperm DNA of adolescent male mice was investigated. Cyclophosphamide and thymoquinone were administered intraperitoneally by a sin...
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| Format: | Article |
| Language: | English |
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Center for Enhancing Knowledge (CEK)
2013
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| Online Access: | http://irep.iium.edu.my/32053/1/EIJST_-_Saheera_et._al.pdf |
| _version_ | 1825647474936643584 |
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| author | Saheera, Kamarzaman Sha'ban, Munirah Abdul Rahman, Suzanah |
| author_facet | Saheera, Kamarzaman Sha'ban, Munirah Abdul Rahman, Suzanah |
| author_sort | Saheera, Kamarzaman |
| collection | IIUM |
| description | The potential of thymoquinone, an active component of Nigella Sativa, in lowering chemotherapeuticassociated
toxicity of cyclophosphamide in the testis, epididymis and sperm DNA of adolescent male mice
was investigated. Cyclophosphamide and thymoquinone were administered intraperitoneally by a single
injection at 200 mg/kg and 10 mg/kg on alternate days, respectively. Effects were observed on days 5, 32
and 53. Results show that thirty-two days following exposure to cyclophosphamide, the spermatogonia cells
was reduced by 52% than that in the control group, p<0.05. Treatment with cyclophosphamide also induced
significant toxicity as shown by hypertrophy in the mean diameter of seminiferous tubules and tubular lumen
(p<0.05), vacuolization of spermatogonial layers and destruction of interstitial tissues. Administration of
thymoquinone exhibited significant reduction in overall toxicity caused by cyclophosphamide. The integrity
of the seminiferous tubule was well preserved and the numbers of total DNA damaged cells were reduced
from 49% to 4% at 32 days. |
| first_indexed | 2024-03-05T23:18:09Z |
| format | Article |
| id | oai:generic.eprints.org:32053 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:18:09Z |
| publishDate | 2013 |
| publisher | Center for Enhancing Knowledge (CEK) |
| record_format | dspace |
| spelling | oai:generic.eprints.org:320532016-02-02T04:57:48Z http://irep.iium.edu.my/32053/ Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone Saheera, Kamarzaman Sha'ban, Munirah Abdul Rahman, Suzanah Q Science (General) R Medicine (General) The potential of thymoquinone, an active component of Nigella Sativa, in lowering chemotherapeuticassociated toxicity of cyclophosphamide in the testis, epididymis and sperm DNA of adolescent male mice was investigated. Cyclophosphamide and thymoquinone were administered intraperitoneally by a single injection at 200 mg/kg and 10 mg/kg on alternate days, respectively. Effects were observed on days 5, 32 and 53. Results show that thirty-two days following exposure to cyclophosphamide, the spermatogonia cells was reduced by 52% than that in the control group, p<0.05. Treatment with cyclophosphamide also induced significant toxicity as shown by hypertrophy in the mean diameter of seminiferous tubules and tubular lumen (p<0.05), vacuolization of spermatogonial layers and destruction of interstitial tissues. Administration of thymoquinone exhibited significant reduction in overall toxicity caused by cyclophosphamide. The integrity of the seminiferous tubule was well preserved and the numbers of total DNA damaged cells were reduced from 49% to 4% at 32 days. Center for Enhancing Knowledge (CEK) 2013-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32053/1/EIJST_-_Saheera_et._al.pdf Saheera, Kamarzaman and Sha'ban, Munirah and Abdul Rahman, Suzanah (2013) Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone. European International Journal of Science and Technology, 2 (7). pp. 119-136. ISSN 2304-9693 http://nu.kku.ac.th/eng/index.php/all-news/92-european-international-journal-of-science-and-technology |
| spellingShingle | Q Science (General) R Medicine (General) Saheera, Kamarzaman Sha'ban, Munirah Abdul Rahman, Suzanah Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone |
| title | Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone |
| title_full | Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone |
| title_fullStr | Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone |
| title_full_unstemmed | Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone |
| title_short | Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone |
| title_sort | effects on mouse spermatogenesis and dna fragmentation following exposure to cyclophosphamide and thymoquinone |
| topic | Q Science (General) R Medicine (General) |
| url | http://irep.iium.edu.my/32053/1/EIJST_-_Saheera_et._al.pdf |
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