Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats
Eurycoma longifolia jack(E.L) root extract has become famous for giving enhanced sexual performance ability through elevation of serum testosterone levels in males. However, although very widely used, it’s possible adverse effects are poorly studied .The objective of this study is to find out if the...
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| Format: | Article |
| Language: | English |
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Asian Economic and Social Society
2013
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| Online Access: | http://irep.iium.edu.my/32062/1/101_jasr_3%288%29843-851.pdf |
| _version_ | 1825647476346978304 |
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| author | Faisal, Ghasak Ghazi Mustafa Al-Ahmad, Basma Ezzat Mustafa, Nazih Shaban Najmuldeen, Ghazi Faisal Althunibat, Osama Yousef Azzubaidi, Marwan Saad |
| author_facet | Faisal, Ghasak Ghazi Mustafa Al-Ahmad, Basma Ezzat Mustafa, Nazih Shaban Najmuldeen, Ghazi Faisal Althunibat, Osama Yousef Azzubaidi, Marwan Saad |
| author_sort | Faisal, Ghasak Ghazi |
| collection | IIUM |
| description | Eurycoma longifolia jack(E.L) root extract has become famous for giving enhanced sexual performance ability through elevation of serum testosterone levels in males. However, although very widely used, it’s possible adverse effects are poorly studied .The objective of this study is to find out if there are any pathological changes that occur in the prostate of rats due to consumption of E.L. root extract. Methodology:45 male rats where used in this study , they were divided into three groups ,group(A) contain 5 rats that represent the control group who did not receive any treatment, group (B) with 20 rats ,received 5mg/Kg twice daily of 100:1 freeze dried water extract of Eurycoma longifolia Jack roots and group (C) with 20 rats received 10mg/kg body weight twice daily of the same extract .The duration of treatment for both groups was 4 weeks. At the end of the experiment, blood was obtained from the rats and serum testosterone level (T) was analyzed. The rats were then terminated and the prostate was removed, weighed and its dimensions measured, then it was processed into haematoxiline and eosin stained slides which were observed under light microscope. Results: Group C showed a significant increase in prostate size, weight and serum T. Histopathological examination of the specimens revealed that there were no significant observable changes in group B in comparison with the control group while group C showed a marked cell proliferation of the prostatic gland lining epithelium that appeared as stratification and intraglandular villus formation of the usual single layer columnar epithelium.Areas of mild dysplastic changes were observed in the lining epithelium characterized by nuclear enlargement and irregularities in the nuclear membrane and hyperchromatism. These dysplastic changes were equivalent to low grade prostatic intraepithelial neoplasia PIN. Conclusion: E.L. root extract can cause abnormal proliferation in the prostatic epithelium and these changes are dose dependant.
Keywords: Eurycoma longifolia jack, Prostate, Rat, Dysplasia, Testosterone. |
| first_indexed | 2024-03-05T23:18:10Z |
| format | Article |
| id | oai:generic.eprints.org:32062 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:18:10Z |
| publishDate | 2013 |
| publisher | Asian Economic and Social Society |
| record_format | dspace |
| spelling | oai:generic.eprints.org:320622013-09-30T05:47:53Z http://irep.iium.edu.my/32062/ Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats Faisal, Ghasak Ghazi Mustafa Al-Ahmad, Basma Ezzat Mustafa, Nazih Shaban Najmuldeen, Ghazi Faisal Althunibat, Osama Yousef Azzubaidi, Marwan Saad R Medicine (General) RB Pathology Eurycoma longifolia jack(E.L) root extract has become famous for giving enhanced sexual performance ability through elevation of serum testosterone levels in males. However, although very widely used, it’s possible adverse effects are poorly studied .The objective of this study is to find out if there are any pathological changes that occur in the prostate of rats due to consumption of E.L. root extract. Methodology:45 male rats where used in this study , they were divided into three groups ,group(A) contain 5 rats that represent the control group who did not receive any treatment, group (B) with 20 rats ,received 5mg/Kg twice daily of 100:1 freeze dried water extract of Eurycoma longifolia Jack roots and group (C) with 20 rats received 10mg/kg body weight twice daily of the same extract .The duration of treatment for both groups was 4 weeks. At the end of the experiment, blood was obtained from the rats and serum testosterone level (T) was analyzed. The rats were then terminated and the prostate was removed, weighed and its dimensions measured, then it was processed into haematoxiline and eosin stained slides which were observed under light microscope. Results: Group C showed a significant increase in prostate size, weight and serum T. Histopathological examination of the specimens revealed that there were no significant observable changes in group B in comparison with the control group while group C showed a marked cell proliferation of the prostatic gland lining epithelium that appeared as stratification and intraglandular villus formation of the usual single layer columnar epithelium.Areas of mild dysplastic changes were observed in the lining epithelium characterized by nuclear enlargement and irregularities in the nuclear membrane and hyperchromatism. These dysplastic changes were equivalent to low grade prostatic intraepithelial neoplasia PIN. Conclusion: E.L. root extract can cause abnormal proliferation in the prostatic epithelium and these changes are dose dependant. Keywords: Eurycoma longifolia jack, Prostate, Rat, Dysplasia, Testosterone. Asian Economic and Social Society 2013-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32062/1/101_jasr_3%288%29843-851.pdf Faisal, Ghasak Ghazi and Mustafa Al-Ahmad, Basma Ezzat and Mustafa, Nazih Shaban and Najmuldeen, Ghazi Faisal and Althunibat, Osama Yousef and Azzubaidi, Marwan Saad (2013) Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats. Journal of Asian Scientific Research, 3 (8). pp. 843-851. ISSN 2223-1331 (Online); 2226-5724 (Print) http://aessweb.com/abstract.php?m=&id=5003Dawda&aid=2234 |
| spellingShingle | R Medicine (General) RB Pathology Faisal, Ghasak Ghazi Mustafa Al-Ahmad, Basma Ezzat Mustafa, Nazih Shaban Najmuldeen, Ghazi Faisal Althunibat, Osama Yousef Azzubaidi, Marwan Saad Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats |
| title | Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats |
| title_full | Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats |
| title_fullStr | Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats |
| title_full_unstemmed | Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats |
| title_short | Histopathological effects of Eurycoma Longifolia jack extract (tongkat ali) on the prostate of rats |
| title_sort | histopathological effects of eurycoma longifolia jack extract tongkat ali on the prostate of rats |
| topic | R Medicine (General) RB Pathology |
| url | http://irep.iium.edu.my/32062/1/101_jasr_3%288%29843-851.pdf |
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