| Summary: | The research was conducted to know the genotype of Limousin cross Madura cattle (Limura) and investigate the effect of polymorphism growth hormone (GH) gene for growth traits. The
growth traits were examined based on quantitative data which contain birth weight and body weight at day 60, average daily gain (ADG), body length, heart girth, withers height and hip height. The sample for the experiment consist of 35 Limura calves and 10 Madura calves. Analysis of polymorphism GH gene were using Polymerase Chain Reaction-Restriction
Fragment Lenght Polymorphism (PCR-RFLP) method. The PCR product of GH gene in this research (211 bp) was digested by AluI enzyme. The relatedness between genotype and growth traits were analyzed using one way ANOVA, where three genotype groups (LL_Limura, LV_Limura, and LL_Madura) as factors. The results indicated that the genotype of Madura cattle were 100% LL and the genotype of Limura cattle were LL (82.86%) and LV (17.14%). There are no significant differences of birth weight and pre-weaning body weight
in three groups of genotype. However, both genotype LL and LV of Limura cattle had greater ADG, body length at birth and pre-weaning hip height gain than those of LL_Madura
genotypes (p<0.05). In conclusion, the polymorphism GH gene does not affect the growth traits, but the differences of two breeds (Limura dan Madura cattle) seem influence the growth trait of the cattle.
Key Words: Genotype, GH gene, Growth traits, Limousin cross Madura cattle (Limura)
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