Amplifikasi DNA Gen Meat Tendernes pada Sapi Bali (Bos sondaicus)

The aim of this study was to know DNA band pattern as a result of amplification using DNA primer from meat tenderness gene of Bali cattle. Sample used in this study was DNA isolated from blood Bali cattle. Blood samples was collected in heparin tubes from jugular vein. Leucocyte cells were isolated...

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Bibliographic Details
Main Author: Hartatik, Tety
Format: Article
Language:English
Published: 2012
Subjects:
Online Access:https://repository.ugm.ac.id/32356/1/22_2012_A_Susilo_et_al__Jurnal_THT.pdf
Description
Summary:The aim of this study was to know DNA band pattern as a result of amplification using DNA primer from meat tenderness gene of Bali cattle. Sample used in this study was DNA isolated from blood Bali cattle. Blood samples was collected in heparin tubes from jugular vein. Leucocyte cells were isolated using RBCs (Red Blood Cells) lysis buffer. To get DNA fragmen, marbling and meat tenderness gene were amplified by using DNA primer for for meat tenderness (forward: 5CTACCGGGACGTCAACCT-3; reverse: 5-GGTTGTCGGGGTAGCTCA-3). The size of DNA fragment were 210 bp, respectively. Key words: Bali cattle, meat tenderness gene