Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia
This study attempted to identify Leu/Val polymorphismof growth hormone gene in Limousin cross local cattle. The DNA samples were collected from Maduracattle (65), Limousin-Madura cattle(81), Ongole Crossbred cattle (PO, 52), and Limousin-PO cattle (56), also Limousin bull semen (6). A 211 bp DNA f...
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| Format: | Article |
| Language: | English |
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2013
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| Online Access: | https://repository.ugm.ac.id/32357/1/23_2013_Hartatik_et_al_Proceedia.pdf |
| _version_ | 1797020108275580928 |
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| author | Hartatik, Tety |
| author_facet | Hartatik, Tety |
| author_sort | Hartatik, Tety |
| collection | UGM |
| description | This study attempted to identify Leu/Val polymorphismof growth hormone gene in Limousin cross local cattle. The
DNA samples were collected from Maduracattle (65), Limousin-Madura cattle(81), Ongole Crossbred cattle (PO, 52), and Limousin-PO cattle (56), also Limousin bull semen (6). A 211 bp DNA fragment of growth hormone gene spanning from the forth intron (49 bp) to five exon (162 bp)was amplified using PCR method and digested with AluI restriction enzyme to identified polymorphism at this locus. Two genotypesLL and LV were observed in PO (96.15%LL, 3.85%LV), Limousin-Madura (81.46%LL, 18.52%LV), Limousin-PO cattle (78.57%LL, 21.43%LV), and Limousin (66.67%LL, 33.33%LV). Madura cattles were found monomorphic for this locus producing only one
genotype LL (100%). The results of this research show thatthe increasing number of genotype leu/val in Limousin
cross local cattle is about 17.58% and 18.52% for Limousin-PO cattle and Limousin-Madura, respectively, compared
to the local cattle (PO and Madura cattle). The allelic frequencies are statistically similar as revealed by Chi-Square test. This research concludes that the polymorphic leu/val of growth hormone gene occurs in Limousine-cross local cattle whereas monomorphic leu/leu of growth hormone gene is found in Madura cattle. Thus, the finding of this
research would be useful as a control for genetic equilibrium in Madura cattle population and to protect uncontrolled crossbreeding. |
| first_indexed | 2024-03-13T19:10:29Z |
| format | Article |
| id | oai:generic.eprints.org:32357 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T19:10:29Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:323572014-08-29T07:48:27Z https://repository.ugm.ac.id/32357/ Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia Hartatik, Tety Husbandry Livestock This study attempted to identify Leu/Val polymorphismof growth hormone gene in Limousin cross local cattle. The DNA samples were collected from Maduracattle (65), Limousin-Madura cattle(81), Ongole Crossbred cattle (PO, 52), and Limousin-PO cattle (56), also Limousin bull semen (6). A 211 bp DNA fragment of growth hormone gene spanning from the forth intron (49 bp) to five exon (162 bp)was amplified using PCR method and digested with AluI restriction enzyme to identified polymorphism at this locus. Two genotypesLL and LV were observed in PO (96.15%LL, 3.85%LV), Limousin-Madura (81.46%LL, 18.52%LV), Limousin-PO cattle (78.57%LL, 21.43%LV), and Limousin (66.67%LL, 33.33%LV). Madura cattles were found monomorphic for this locus producing only one genotype LL (100%). The results of this research show thatthe increasing number of genotype leu/val in Limousin cross local cattle is about 17.58% and 18.52% for Limousin-PO cattle and Limousin-Madura, respectively, compared to the local cattle (PO and Madura cattle). The allelic frequencies are statistically similar as revealed by Chi-Square test. This research concludes that the polymorphic leu/val of growth hormone gene occurs in Limousine-cross local cattle whereas monomorphic leu/leu of growth hormone gene is found in Madura cattle. Thus, the finding of this research would be useful as a control for genetic equilibrium in Madura cattle population and to protect uncontrolled crossbreeding. 2013 Article NonPeerReviewed application/pdf en https://repository.ugm.ac.id/32357/1/23_2013_Hartatik_et_al_Proceedia.pdf Hartatik, Tety (2013) Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia. Procedia Environmental Sciences 17 . |
| spellingShingle | Husbandry Livestock Hartatik, Tety Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia |
| title | Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia |
| title_full | Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia |
| title_fullStr | Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia |
| title_full_unstemmed | Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia |
| title_short | Polymorphism leu/val of Growth Hormone Gene Identified from Limousin Cross Local Cattle in Indonesia |
| title_sort | polymorphism leu val of growth hormone gene identified from limousin cross local cattle in indonesia |
| topic | Husbandry Livestock |
| url | https://repository.ugm.ac.id/32357/1/23_2013_Hartatik_et_al_Proceedia.pdf |
| work_keys_str_mv | AT hartatiktety polymorphismleuvalofgrowthhormonegeneidentifiedfromlimousincrosslocalcattleinindonesia |