| Summary: | Toxoplasmosis is a crucial public health problem because of its high economic and social impact in relation to cost of patient care, born defect such as hydrocephalus, mental retardation, retinochoriditis, and blindness in children. Environmental risk factors that likely increase the spread of toxoplasmosis in the Special Province of Yogyakarta and Bali are the presence of definitive host in the population, altitude, land surface temparature and climate condition that enable the development of oocyist, high prevalance of toxoplasmosis in goats and cows (49% and 21%), eating habits and non chlorinated water. The aims of this research is to study serological prevalence of oxoplasmosis in the population and get an overview of association between risk factors and toxoplasmosis infection, comprising gender, age, geography, contact with cats, eating habits, occupation related to contact with raw meat, water used to cook and activities related to contact with soil through EcoHealth approaches. The amount of 1050 serum samples were collected using double cluster design from Special Province of Yogyakarta and 960 serum samples from Bali. Data of risk factors were obtained through interview with respondents or parents of respondents. Anti toxoplasmosis IgG and IgM detected with ELISA method using recombinant protein of GRA-1 tachizoites of local isolate Toxoplasma gondii as an antigen. Positive and negative control used human seru that had been positively or negatively diagnosed at Dr. Sardjito, Bethesda and Panti Rapih Hospital Yogyakarta. Risk factors were analyzed their significance and odds ratio at confidence interval 95%, and mapping of serological prevalence with Geographical Information System. Serological prevalence of toxoplasmosis at the Special Province of Yogyakarta was 61,5%. Highest at District of Kulon Progo (78,6%), later at District of Sleman (72,4%), at the City of Yogyakarta (69,5%), at District of Bantul (57,6%) and at District of Gunung Kidul (29,5%) and Bali (39%). Risk factors significantly associated with serological prevalence of toxoplasmosis at the Yogyakarta Special Province examination were gender, ad for residence plain (geography), contact with cat, undercooked goat meat consumption, raw vegetable consumption at food stalls, occupation related to contact with raw meat, and occupation/activities related to contact with soil. Age, undercooked chicken and beef meat consumption, raw vegetable consumption at home and water used to cook were insignificantly associated with serological prevalence of toxoplosmosis.
Keywords: Toxoplasmosis, IgM, IgG, GRA-1recombinant protein, ELISA, EcoHealth
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