Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)

The present study was conducted to assess the antioxidant properties of ethanol, ethyl acetate, methanol and hexane extracts from leaves of Pereskia bleo using 2,2-diphenyl-1-picrylhydrazyl method (DPPH assay), ferric reducing antioxidant power (FRAP assay) and β-Carotene-Linoleic acid methods. Tota...

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Main Authors: Hassanbaglou, Behnaz, Abdul Hamid, Azizah, A. M., Roheeyati, Mohd Saleh, Norihan, Abdulamir, AhmadSahib, Khatib, Alfi, Sabu, MC
Format: Article
Language:English
Published: Academic Journals 2012
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Online Access:http://irep.iium.edu.my/33294/1/Hassanbaglou_et_al_%282012%29.pdf
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author Hassanbaglou, Behnaz
Abdul Hamid, Azizah
A. M., Roheeyati
Mohd Saleh, Norihan
Abdulamir, AhmadSahib
Khatib, Alfi
Sabu, MC
author_facet Hassanbaglou, Behnaz
Abdul Hamid, Azizah
A. M., Roheeyati
Mohd Saleh, Norihan
Abdulamir, AhmadSahib
Khatib, Alfi
Sabu, MC
author_sort Hassanbaglou, Behnaz
collection IIUM
description The present study was conducted to assess the antioxidant properties of ethanol, ethyl acetate, methanol and hexane extracts from leaves of Pereskia bleo using 2,2-diphenyl-1-picrylhydrazyl method (DPPH assay), ferric reducing antioxidant power (FRAP assay) and β-Carotene-Linoleic acid methods. Total phenolic compounds (TPC) and flavonoids of the leaves of P. bleo were measured using Folin-Ciocalteu and HPLC methods respectively. Results of the present study showed that Ethyl acetate extract of P. bleo leaf exhibited significantly (p < 0.05) higher antioxidant activity as measured using DPPH free radical (IC50), FRAP and β-carotene-linoleic assays than that of hexane extract, methanol extract and ethanol extract. Pereskia bleo leaf was also found to contain high amounts of bioactive compounds including total phenolic compounds (109.43 ±0.84 mg GAE/g), epicatechin (575± 0.04 mg/100g), quercetin (110.94 ±0.12 mg/100g), catechin (918 ± 0.01 mg/100 g) and myricetin (9.49±0.55 mg/100g) while the concentrations of α- tocopherol, β-carotene and lycopene were found to be higher (69±0.25, 51.97±1.18 mg/100 g, 92.46±0.41 μg/g), respectively.
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spelling oai:generic.eprints.org:332942013-12-13T08:34:15Z http://irep.iium.edu.my/33294/ Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae) Hassanbaglou, Behnaz Abdul Hamid, Azizah A. M., Roheeyati Mohd Saleh, Norihan Abdulamir, AhmadSahib Khatib, Alfi Sabu, MC QD Chemistry The present study was conducted to assess the antioxidant properties of ethanol, ethyl acetate, methanol and hexane extracts from leaves of Pereskia bleo using 2,2-diphenyl-1-picrylhydrazyl method (DPPH assay), ferric reducing antioxidant power (FRAP assay) and β-Carotene-Linoleic acid methods. Total phenolic compounds (TPC) and flavonoids of the leaves of P. bleo were measured using Folin-Ciocalteu and HPLC methods respectively. Results of the present study showed that Ethyl acetate extract of P. bleo leaf exhibited significantly (p < 0.05) higher antioxidant activity as measured using DPPH free radical (IC50), FRAP and β-carotene-linoleic assays than that of hexane extract, methanol extract and ethanol extract. Pereskia bleo leaf was also found to contain high amounts of bioactive compounds including total phenolic compounds (109.43 ±0.84 mg GAE/g), epicatechin (575± 0.04 mg/100g), quercetin (110.94 ±0.12 mg/100g), catechin (918 ± 0.01 mg/100 g) and myricetin (9.49±0.55 mg/100g) while the concentrations of α- tocopherol, β-carotene and lycopene were found to be higher (69±0.25, 51.97±1.18 mg/100 g, 92.46±0.41 μg/g), respectively. Academic Journals 2012-04-23 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33294/1/Hassanbaglou_et_al_%282012%29.pdf Hassanbaglou, Behnaz and Abdul Hamid, Azizah and A. M., Roheeyati and Mohd Saleh, Norihan and Abdulamir, AhmadSahib and Khatib, Alfi and Sabu, MC (2012) Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae). Journal of Medicinal Plants Research, 6 (15). pp. 2932-2937. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR/edition/23_April,_2012 10.5897/JMPR11.760
spellingShingle QD Chemistry
Hassanbaglou, Behnaz
Abdul Hamid, Azizah
A. M., Roheeyati
Mohd Saleh, Norihan
Abdulamir, AhmadSahib
Khatib, Alfi
Sabu, MC
Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_full Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_fullStr Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_full_unstemmed Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_short Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_sort antioxidant activity of different extracts from leaves of pereskia bleo cactaceae
topic QD Chemistry
url http://irep.iium.edu.my/33294/1/Hassanbaglou_et_al_%282012%29.pdf
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