Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essent...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2012
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| Online Access: | http://irep.iium.edu.my/33298/1/Shamloo_et_al._2012.pdf |
| _version_ | 1825647639619698688 |
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| author | Shamloo, M. Bakar , Jamilah Mat Hashim, D. Khatib, Alfi |
| author_facet | Shamloo, M. Bakar , Jamilah Mat Hashim, D. Khatib, Alfi |
| author_sort | Shamloo, M. |
| collection | IIUM |
| description | The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity,
and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH),
Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essential amino
acids to non-essential amino acids increased after hydrolysis in all samples; however, no significant differences among them were observed. AH had a highest (P < 0.05) DPPH radical-scavenging activity, but no significant
difference in the DPPH between FH and PH was observed. SDS-PAGE patterns for all the hydrolysates showed significant (P < 0.05) reduction in the number and the intensity of the bands with increasing time of hydrolysis. Flavourzyme showed the lowest rate of hydrolytic activity towards the tilapia mince. |
| first_indexed | 2024-03-05T23:20:34Z |
| format | Article |
| id | oai:generic.eprints.org:33298 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:20:34Z |
| publishDate | 2012 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | oai:generic.eprints.org:332982013-12-13T04:19:45Z http://irep.iium.edu.my/33298/ Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates Shamloo, M. Bakar , Jamilah Mat Hashim, D. Khatib, Alfi S Agriculture (General) The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essential amino acids to non-essential amino acids increased after hydrolysis in all samples; however, no significant differences among them were observed. AH had a highest (P < 0.05) DPPH radical-scavenging activity, but no significant difference in the DPPH between FH and PH was observed. SDS-PAGE patterns for all the hydrolysates showed significant (P < 0.05) reduction in the number and the intensity of the bands with increasing time of hydrolysis. Flavourzyme showed the lowest rate of hydrolytic activity towards the tilapia mince. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012-01-28 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33298/1/Shamloo_et_al._2012.pdf Shamloo, M. and Bakar , Jamilah and Mat Hashim, D. and Khatib, Alfi (2012) Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates. International Food Research Journal, 19 (1). pp. 183-188. ISSN 19854668 (P), 22317546 (O) http://www.ifrj.upm.edu.my/ |
| spellingShingle | S Agriculture (General) Shamloo, M. Bakar , Jamilah Mat Hashim, D. Khatib, Alfi Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_full | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_fullStr | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_full_unstemmed | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_short | Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates |
| title_sort | biochemical properties of red tilapia oreochromis niloticus protein hydrolysates |
| topic | S Agriculture (General) |
| url | http://irep.iium.edu.my/33298/1/Shamloo_et_al._2012.pdf |
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