Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin
Individual iso-α-acids that are responsible for the bitter taste of beer need to be isolated because these acids are required as reference standards in quantitative analysis and when studying the parameters which effect the quality of beer. However, these pure compounds are very expensive, due to in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010
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| Online Access: | http://irep.iium.edu.my/33381/1/Khatib_et_al._2010_FC.pdf |
| _version_ | 1825647648766427136 |
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| author | Khatib, Alfi Wilson, Erica G. Supardi, Moses Verpoorte, Robert |
| author_facet | Khatib, Alfi Wilson, Erica G. Supardi, Moses Verpoorte, Robert |
| author_sort | Khatib, Alfi |
| collection | IIUM |
| description | Individual iso-α-acids that are responsible for the bitter taste of beer need to be isolated because these acids are required as reference standards in quantitative analysis and when studying the parameters which effect the quality of beer. However, these pure compounds are very expensive, due to inefficient isolation methods. In this study a new isolation method has been developed, in order to reduce the isolation cost. β-Cyclodextrin has been used for the isolation of trans- and cis-iso-α-acids. The separation from the mixture of stereoisomers was achieved by complexation, using ethanol:water (1:2, v/v) as a solvent at a temperature of 50 °C for 30 min. The molar ratio of iso-α-acids sample to β-cyclodextrin for complexation was 1:1. Precipitation time varied between 9 h and 2 days, depending on the iso-α-acid. Release of the guest from the cyclodextrin complex was successfully accomplished by elution with methanol. |
| first_indexed | 2024-03-05T23:20:43Z |
| format | Article |
| id | oai:generic.eprints.org:33381 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:20:43Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:generic.eprints.org:333812013-12-16T06:12:50Z http://irep.iium.edu.my/33381/ Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin Khatib, Alfi Wilson, Erica G. Supardi, Moses Verpoorte, Robert QD Chemistry Individual iso-α-acids that are responsible for the bitter taste of beer need to be isolated because these acids are required as reference standards in quantitative analysis and when studying the parameters which effect the quality of beer. However, these pure compounds are very expensive, due to inefficient isolation methods. In this study a new isolation method has been developed, in order to reduce the isolation cost. β-Cyclodextrin has been used for the isolation of trans- and cis-iso-α-acids. The separation from the mixture of stereoisomers was achieved by complexation, using ethanol:water (1:2, v/v) as a solvent at a temperature of 50 °C for 30 min. The molar ratio of iso-α-acids sample to β-cyclodextrin for complexation was 1:1. Precipitation time varied between 9 h and 2 days, depending on the iso-α-acid. Release of the guest from the cyclodextrin complex was successfully accomplished by elution with methanol. Elsevier 2010-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33381/1/Khatib_et_al._2010_FC.pdf Khatib, Alfi and Wilson, Erica G. and Supardi, Moses and Verpoorte, Robert (2010) Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin. Food Chemistry, 119 (1). pp. 354-357. ISSN 0308-8146 http://www.sciencedirect.com/science/article/pii/S0308814609008085 10.1016/j.foodchem.2009.06.011 |
| spellingShingle | QD Chemistry Khatib, Alfi Wilson, Erica G. Supardi, Moses Verpoorte, Robert Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin |
| title | Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin |
| title_full | Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin |
| title_fullStr | Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin |
| title_full_unstemmed | Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin |
| title_short | Isolation of individual hop iso-α-acids stereoisomers by β-cyclodextrin |
| title_sort | isolation of individual hop iso α acids stereoisomers by β cyclodextrin |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/33381/1/Khatib_et_al._2010_FC.pdf |
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