High performance liquid chromatographic method for Iso-a-Acids
Using pure trans/cis-isocohumulone, trans/cis-isohumulone, and trans/cisisoadhumulone an isocratic HPLC system has been developed for quantitation of these compounds in hop extracts and beers. The mobile phase contains acetonitrile-water- H3PO4 (50 : 50 : 0.01, v/v/v) and is used with a Phenomene...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2006
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/33743/1/Khatib_et_al%2C_2006._JLCRT.pdf |
| _version_ | 1825647697330176000 |
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| author | Khatib, Alfi Kim, Hye Kyong Wilson, Erica G. Verpoorte, Robert |
| author_facet | Khatib, Alfi Kim, Hye Kyong Wilson, Erica G. Verpoorte, Robert |
| author_sort | Khatib, Alfi |
| collection | IIUM |
| description | Using pure trans/cis-isocohumulone, trans/cis-isohumulone, and trans/cisisoadhumulone
an isocratic HPLC system has been developed for quantitation of these
compounds in hop extracts and beers. The mobile phase contains acetonitrile-water-
H3PO4 (50 : 50 : 0.01, v/v/v) and is used with a Phenomenex Hypersil 5 m
C18 column 250 � 4.6 mm, flow rate 1.5mL/min. Baseline separation of all 6
isomers was achieved with a total run time of 25 min. The UV spectrum of these
pure compounds using this system are quite different. The effect of different mobile
phase compositions on separation was investigated as well as the chromatographic
parameters, detection limit, and linearity. |
| first_indexed | 2024-03-05T23:21:26Z |
| format | Article |
| id | oai:generic.eprints.org:33743 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:21:26Z |
| publishDate | 2006 |
| publisher | Taylor & Francis Group |
| record_format | dspace |
| spelling | oai:generic.eprints.org:337432014-01-06T04:19:33Z http://irep.iium.edu.my/33743/ High performance liquid chromatographic method for Iso-a-Acids Khatib, Alfi Kim, Hye Kyong Wilson, Erica G. Verpoorte, Robert QD Chemistry Using pure trans/cis-isocohumulone, trans/cis-isohumulone, and trans/cisisoadhumulone an isocratic HPLC system has been developed for quantitation of these compounds in hop extracts and beers. The mobile phase contains acetonitrile-water- H3PO4 (50 : 50 : 0.01, v/v/v) and is used with a Phenomenex Hypersil 5 m C18 column 250 � 4.6 mm, flow rate 1.5mL/min. Baseline separation of all 6 isomers was achieved with a total run time of 25 min. The UV spectrum of these pure compounds using this system are quite different. The effect of different mobile phase compositions on separation was investigated as well as the chromatographic parameters, detection limit, and linearity. Taylor & Francis Group 2006-04-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33743/1/Khatib_et_al%2C_2006._JLCRT.pdf Khatib, Alfi and Kim, Hye Kyong and Wilson, Erica G. and Verpoorte, Robert (2006) High performance liquid chromatographic method for Iso-a-Acids. Journal of Liquid Chromatography & Related Technologies, 29 (2). pp. 293-302. ISSN 1082-6076 print/1520-572X online http://www.tandfonline.com/doi/abs/10.1080/10826070500416676?journalCode=ljlc20#preview 10.1080/10826070500416676 |
| spellingShingle | QD Chemistry Khatib, Alfi Kim, Hye Kyong Wilson, Erica G. Verpoorte, Robert High performance liquid chromatographic method for Iso-a-Acids |
| title | High performance liquid chromatographic method for Iso-a-Acids |
| title_full | High performance liquid chromatographic method for Iso-a-Acids |
| title_fullStr | High performance liquid chromatographic method for Iso-a-Acids |
| title_full_unstemmed | High performance liquid chromatographic method for Iso-a-Acids |
| title_short | High performance liquid chromatographic method for Iso-a-Acids |
| title_sort | high performance liquid chromatographic method for iso a acids |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/33743/1/Khatib_et_al%2C_2006._JLCRT.pdf |
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