Cloning and expression of Voltage-dependent Anion Channel 2 (VDAC2) from electrically stimulated chicken
VDAC2 protein identified to be overexpressed in stunned chicken is a potential biomarker in differentiating stunned chicken since the expression was proportional to the voltage applied. To facilitate research, the gene encoding VDAC is cloned, expressed and optimized in BL21-AI Escherichia coli....
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2013
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| Online Access: | http://irep.iium.edu.my/34096/1/JPAM_VDAC2.pdf |
| _version_ | 1825647732682915840 |
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| author | Ahmad Hassan, Nurhidayah Amid, Azura Mohd. Salleh, Hamzah |
| author_facet | Ahmad Hassan, Nurhidayah Amid, Azura Mohd. Salleh, Hamzah |
| author_sort | Ahmad Hassan, Nurhidayah |
| collection | IIUM |
| description | VDAC2 protein identified to be overexpressed in stunned chicken is a potential
biomarker in differentiating stunned chicken since the expression was proportional to
the voltage applied. To facilitate research, the gene encoding VDAC is cloned, expressed
and optimized in BL21-AI Escherichia coli. cDNA was synthesized from mRNA of stunned
chicken’s skeletal muscle and cloned using recombination reaction. All positive clones
were identified by PCR and restriction enzyme digestion as well as DNA sequencing.
Recombinant VDAC2 protein was purified by Ni-NTA spin-column. Western blot performed
using polyclonal anti-N terminal human VDAC2 and anti-His tag revealed a 34 kDa
protein, confirming the expression of recombinant VDAC2. |
| first_indexed | 2024-03-05T23:21:57Z |
| format | Article |
| id | oai:generic.eprints.org:34096 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:21:57Z |
| publishDate | 2013 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | dspace |
| spelling | oai:generic.eprints.org:340962021-07-14T09:46:50Z http://irep.iium.edu.my/34096/ Cloning and expression of Voltage-dependent Anion Channel 2 (VDAC2) from electrically stimulated chicken Ahmad Hassan, Nurhidayah Amid, Azura Mohd. Salleh, Hamzah T175 Industrial research. Research and development VDAC2 protein identified to be overexpressed in stunned chicken is a potential biomarker in differentiating stunned chicken since the expression was proportional to the voltage applied. To facilitate research, the gene encoding VDAC is cloned, expressed and optimized in BL21-AI Escherichia coli. cDNA was synthesized from mRNA of stunned chicken’s skeletal muscle and cloned using recombination reaction. All positive clones were identified by PCR and restriction enzyme digestion as well as DNA sequencing. Recombinant VDAC2 protein was purified by Ni-NTA spin-column. Western blot performed using polyclonal anti-N terminal human VDAC2 and anti-His tag revealed a 34 kDa protein, confirming the expression of recombinant VDAC2. Journal of Pure and Applied Microbiology 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34096/1/JPAM_VDAC2.pdf Ahmad Hassan, Nurhidayah and Amid, Azura and Mohd. Salleh, Hamzah (2013) Cloning and expression of Voltage-dependent Anion Channel 2 (VDAC2) from electrically stimulated chicken. Journal of Pure and Applied Microbiology, 7 (Spec.). pp. 43-52. ISSN 0973-7510 http://www.microbiologyjournal.org/jmabsread.php?snoid=1420&month=Nov&year=2014 |
| spellingShingle | T175 Industrial research. Research and development Ahmad Hassan, Nurhidayah Amid, Azura Mohd. Salleh, Hamzah Cloning and expression of Voltage-dependent Anion Channel 2 (VDAC2) from electrically stimulated chicken |
| title | Cloning and expression of Voltage-dependent Anion
Channel 2 (VDAC2) from electrically stimulated chicken |
| title_full | Cloning and expression of Voltage-dependent Anion
Channel 2 (VDAC2) from electrically stimulated chicken |
| title_fullStr | Cloning and expression of Voltage-dependent Anion
Channel 2 (VDAC2) from electrically stimulated chicken |
| title_full_unstemmed | Cloning and expression of Voltage-dependent Anion
Channel 2 (VDAC2) from electrically stimulated chicken |
| title_short | Cloning and expression of Voltage-dependent Anion
Channel 2 (VDAC2) from electrically stimulated chicken |
| title_sort | cloning and expression of voltage dependent anion channel 2 vdac2 from electrically stimulated chicken |
| topic | T175 Industrial research. Research and development |
| url | http://irep.iium.edu.my/34096/1/JPAM_VDAC2.pdf |
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