Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion
The study aimed to produce lipase using abundant raw material in Malaysia employing a cost- effective method which is solid-state bioconversion (SSB). The optimization of the production was achieved with 400 U/gds which was more than 4-folds compared to the screening experiments. Characterizatio...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/35098/1/AICHE-amal.PDF |
| _version_ | 1825647840691486720 |
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| author | Alam, Md. Zahangir Elgharbawy, Amal Ahmed Hamzah, Mohd. Salleh |
| author_facet | Alam, Md. Zahangir Elgharbawy, Amal Ahmed Hamzah, Mohd. Salleh |
| author_sort | Alam, Md. Zahangir |
| collection | IIUM |
| description | The study aimed to produce lipase using abundant raw material in Malaysia employing a cost- effective
method which is solid-state bioconversion (SSB). The optimization of the production was achieved with
400 U/gds which was more than 4-folds compared to the screening experiments. Characterization of the
produced lipase showed promising results in terms of stability as well as in the hydrolysis of lipids. The
enzyme was stable at 50-55°, contact time of one hour and hydrolyzed reaction to 50% free fatty acids. |
| first_indexed | 2024-03-05T23:23:32Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:35098 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:23:32Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:350982021-06-29T03:09:52Z http://irep.iium.edu.my/35098/ Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion Alam, Md. Zahangir Elgharbawy, Amal Ahmed Hamzah, Mohd. Salleh TP248.13 Biotechnology The study aimed to produce lipase using abundant raw material in Malaysia employing a cost- effective method which is solid-state bioconversion (SSB). The optimization of the production was achieved with 400 U/gds which was more than 4-folds compared to the screening experiments. Characterization of the produced lipase showed promising results in terms of stability as well as in the hydrolysis of lipids. The enzyme was stable at 50-55°, contact time of one hour and hydrolyzed reaction to 50% free fatty acids. 2013 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/35098/1/AICHE-amal.PDF Alam, Md. Zahangir and Elgharbawy, Amal Ahmed and Hamzah, Mohd. Salleh (2013) Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion. In: 2013 AIChE Annual Meeting, 3-8 November 2013, San Fransisco, USA. |
| spellingShingle | TP248.13 Biotechnology Alam, Md. Zahangir Elgharbawy, Amal Ahmed Hamzah, Mohd. Salleh Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion |
| title | Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion |
| title_full | Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion |
| title_fullStr | Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion |
| title_full_unstemmed | Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion |
| title_short | Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion |
| title_sort | optimization and characterization of candida cylindracea lipase produced from palm kernel cake by solid state bioconversion |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/35098/1/AICHE-amal.PDF |
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