The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation
Different microelectrode designs for a dielectrophoresis (DEP)-based lab-on-chip, have significant effect on the DEP force produced. Study on the microelectrode factor is essential, as the geometry and the numbers of microelectrode can influence particles and/or cells polarization. This paper presen...
| Main Authors: | , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
IEEE Xplore
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/36109/1/The_octupole.pdf |
| _version_ | 1796878696477360128 |
|---|---|
| author | Ibrahim, Siti Noorjannah Alkaisi, Maan M. |
| author_facet | Ibrahim, Siti Noorjannah Alkaisi, Maan M. |
| author_sort | Ibrahim, Siti Noorjannah |
| collection | IIUM |
| description | Different microelectrode designs for a dielectrophoresis (DEP)-based lab-on-chip, have significant effect on the DEP force produced. Study on the microelectrode factor is essential, as the geometry and the numbers of microelectrode can influence particles and/or cells polarization. This paper presents one of the three new microelectrode designs, called the octopule microelectrode, in a study on the microelectrode factor of the DEP trapping force. The octupole pattern was constructed on a metal-insulator-metal layer structured on a Silicon Nitride(Si3N4) coated Silicon(Si) substrate. The first layer or back contact is made from a 20nm Nickel-Chromium(NiCr) and a 100nm gold (Au). Then, an insulator made of SU-8-2005 was spin-coated on the metal layer to create arrays of microcavities or cell traps. The third layer, where the octupole geometry was patterned, consists of 20nm NiCr and 100nm Au layers. The microcavities which were defined on the SU-8 layer, allows access to the back contact. Gradient of electric fields which represent the actual DEP trapping regions were profiled using COMSOL Multiphysics 3.5a software. Then, the microelectrode trapping ability was evaluated using polystyrene microbeads suspended in deionised (DI) water as the cell model. Results obtained from the experiment were in agreement with results from simulation studies where polystyrene microbeads concentrated at the trapping region and filled the microcavity. |
| first_indexed | 2024-03-05T23:25:12Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:36109 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:25:12Z |
| publishDate | 2012 |
| publisher | IEEE Xplore |
| record_format | dspace |
| spelling | oai:generic.eprints.org:361092015-12-09T09:37:05Z http://irep.iium.edu.my/36109/ The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation Ibrahim, Siti Noorjannah Alkaisi, Maan M. TK Electrical engineering. Electronics Nuclear engineering Different microelectrode designs for a dielectrophoresis (DEP)-based lab-on-chip, have significant effect on the DEP force produced. Study on the microelectrode factor is essential, as the geometry and the numbers of microelectrode can influence particles and/or cells polarization. This paper presents one of the three new microelectrode designs, called the octopule microelectrode, in a study on the microelectrode factor of the DEP trapping force. The octupole pattern was constructed on a metal-insulator-metal layer structured on a Silicon Nitride(Si3N4) coated Silicon(Si) substrate. The first layer or back contact is made from a 20nm Nickel-Chromium(NiCr) and a 100nm gold (Au). Then, an insulator made of SU-8-2005 was spin-coated on the metal layer to create arrays of microcavities or cell traps. The third layer, where the octupole geometry was patterned, consists of 20nm NiCr and 100nm Au layers. The microcavities which were defined on the SU-8 layer, allows access to the back contact. Gradient of electric fields which represent the actual DEP trapping regions were profiled using COMSOL Multiphysics 3.5a software. Then, the microelectrode trapping ability was evaluated using polystyrene microbeads suspended in deionised (DI) water as the cell model. Results obtained from the experiment were in agreement with results from simulation studies where polystyrene microbeads concentrated at the trapping region and filled the microcavity. IEEE Xplore 2012-09-19 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/36109/1/The_octupole.pdf Ibrahim, Siti Noorjannah and Alkaisi, Maan M. (2012) The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation. In: 10th IEEE International Conference on Semiconductor Electronics, ICSE 2012, 19-21 September 2012, Kuala Lumpur, Malaysia. http://ieeemalaysia-eds.org/icse2012/ 10.1109/SMElec.2012.6417105 |
| spellingShingle | TK Electrical engineering. Electronics Nuclear engineering Ibrahim, Siti Noorjannah Alkaisi, Maan M. The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation |
| title | The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation
|
| title_full | The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation
|
| title_fullStr | The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation
|
| title_full_unstemmed | The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation
|
| title_short | The octupole microelectrode for dielectrophoretic trapping of single cells-design and simulation
|
| title_sort | octupole microelectrode for dielectrophoretic trapping of single cells design and simulation |
| topic | TK Electrical engineering. Electronics Nuclear engineering |
| url | http://irep.iium.edu.my/36109/1/The_octupole.pdf |
| work_keys_str_mv | AT ibrahimsitinoorjannah theoctupolemicroelectrodefordielectrophoretictrappingofsinglecellsdesignandsimulation AT alkaisimaanm theoctupolemicroelectrodefordielectrophoretictrappingofsinglecellsdesignandsimulation AT ibrahimsitinoorjannah octupolemicroelectrodefordielectrophoretictrappingofsinglecellsdesignandsimulation AT alkaisimaanm octupolemicroelectrodefordielectrophoretictrappingofsinglecellsdesignandsimulation |