Potential halophilic cellulases for in situ enzymatic saccharification of ionic liquids pretreated lignocelluloses
Ionic liquids (ILs) have been used as an alternative green solvent for lignocelluloses pretreatment. However, being a salt, ILs exhibit an inhibitory effect on cellulases activity, thus making the subsequent saccharification inefficient. The aim of the present study is to produce salt-tolerant cel...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/36181/1/Bioresource_paper_-main.pdf |
| Summary: | Ionic liquids (ILs) have been used as an alternative green solvent for lignocelluloses pretreatment.
However, being a salt, ILs exhibit an inhibitory effect on cellulases activity, thus making the subsequent
saccharification inefficient. The aim of the present study is to produce salt-tolerant cellulases, with the
rationale that the enzyme also tolerant to the presence of ILs. The enzyme was produced from a locally
isolated halophilic strain and was characterized and assessed for its tolerance to different types of ionic
liquids. The results showed that halophilic cellulases produced from Aspergillus terreus UniMAP AA-6
exhibited higher tolerance to ILs and enhanced thermo stability in the presence of high saline conditions. |
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