Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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AENSI Publications
2014
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| Online Access: | http://irep.iium.edu.my/36413/1/734-743_Parveen_.pdf http://irep.iium.edu.my/36413/4/36413_Development%20and%20improvement%20of%20anti-gout_SCOPUS.pdf |
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| author | Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun |
| author_facet | Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun |
| author_sort | Jamal, Parveen |
| collection | IIUM |
| description | Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of
hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric
acid leads to a type of arthritis known as gout. In this study, development of process
conditions for XO inhibitory activity from the leaves of Morinda elliptica was
performed by using 70% methanol. Optimization of process parameters such as
extraction temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of
sample to solvent (1g/ml) at five levels was carried out using central composite design
(CCD) for the improvement of activity to treat gout. The analysis of variance
demonstrated that the model F-value of 18.31 showed the significance of the model
with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity
was improved at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized
conditions were verified and the percentage of XO inhibitory activity obtained was
88.93%. The results are encouraging to formulate food, nutraceutical or pharmaceutical
products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to
irresponsive synthetic XOI. |
| first_indexed | 2024-03-05T23:25:49Z |
| format | Article |
| id | oai:generic.eprints.org:36413 |
| institution | International Islamic University Malaysia |
| language | English English |
| last_indexed | 2024-03-05T23:25:49Z |
| publishDate | 2014 |
| publisher | AENSI Publications |
| record_format | dspace |
| spelling | oai:generic.eprints.org:364132021-07-23T02:14:37Z http://irep.iium.edu.my/36413/ Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun T Technology (General) TP248.13 Biotechnology Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from the leaves of Morinda elliptica was performed by using 70% methanol. Optimization of process parameters such as extraction temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of sample to solvent (1g/ml) at five levels was carried out using central composite design (CCD) for the improvement of activity to treat gout. The analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity was improved at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized conditions were verified and the percentage of XO inhibitory activity obtained was 88.93%. The results are encouraging to formulate food, nutraceutical or pharmaceutical products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to irresponsive synthetic XOI. AENSI Publications 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36413/1/734-743_Parveen_.pdf application/pdf en http://irep.iium.edu.my/36413/4/36413_Development%20and%20improvement%20of%20anti-gout_SCOPUS.pdf Jamal, Parveen and Azmi, Saiful Mohammad Nizam and Amid, Azura and Mohd. Salleh, Hamzah and Hashim, Yumi Zuhanis Has-Yun (2014) Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design. Advances in Environmental Biology, 8 (3). pp. 734-743. ISSN 1998-1066 (O), 1995-0756 (P) http://www.aensiweb.com/old/aeb/2014/734-743.pdf |
| spellingShingle | T Technology (General) TP248.13 Biotechnology Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_full | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_fullStr | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_full_unstemmed | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_short | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_sort | development and improvement of anti gout property from aqueous methanol extract of morinda elliptica using central composite design |
| topic | T Technology (General) TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/36413/1/734-743_Parveen_.pdf http://irep.iium.edu.my/36413/4/36413_Development%20and%20improvement%20of%20anti-gout_SCOPUS.pdf |
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