The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo
Background: Antiepileptic drug (phenytoin) is extensively used by pregnant women who have seizure disorders. It is reported to produce a variety of facial defects, and considered as an important cause of nongenetic cleft palate in their offspring. Objective: To investigate the influence of admini...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Department of Anatomy, National University of Singapore
2014
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/36841/1/Influence_of_Ante-Natal_Phenytoin_Therapy.pdf |
| _version_ | 1796878801167187968 |
|---|---|
| author | Khaleel, Ruwaidah F. Selman, Mohammad O. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. |
| author_facet | Khaleel, Ruwaidah F. Selman, Mohammad O. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. |
| author_sort | Khaleel, Ruwaidah F. |
| collection | IIUM |
| description | Background: Antiepileptic drug (phenytoin) is extensively used by pregnant women who have seizure
disorders. It is reported to produce a variety of facial defects, and considered as an important cause of nongenetic
cleft palate in their offspring.
Objective: To investigate the influence of administrating phenytoin to pregnant rats on the process of palatal
fusion in rat embryos.
Material & Method: Female pregnant rats (Rattus norvegicus) were divided into two groups: The control
group (G1) and experimental group (G2). Each group consist of (30) pregnant rats. Group 2 are subdivided
into two subgroups (G2A), (G2B), each group contain 15 pregnant female rats. Each rat was given a dose of
15 mg daily phenytoin was suspended in distilled water to obtain a concentration of 15mg/kg/day , and 0.1 mg/
kg that contain 3mg/rat/day was given I/P. From (E19-E16) of gestation when mothers of G2A reach (E20) of
gestation cesarean section done and weighted embryos and cut the head of embryos, however when mothers
of G2B reach (E21) of gestation normal delivery occur and weighted embryos and cut the head of embryos of
gestation and 50 embryo from each group was used for histological preparation.
Result: At embryonic day 20 (E20) and day 21 (E21), there was delay union of palatal shelves, cartilaginous
structure persist and was longer Phenytoin treated group than normals.
Conclusion: The present study did not demonstrate clefting of the palate in the phenytoin treated group.
However, the histological study of the embryonic development of the palate and its morphogenesis has
demonstrated that progression of fusion is taking place but with a delay in the midline seam disappearance. |
| first_indexed | 2024-03-05T23:26:41Z |
| format | Article |
| id | oai:generic.eprints.org:36841 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:26:41Z |
| publishDate | 2014 |
| publisher | Department of Anatomy, National University of Singapore |
| record_format | dspace |
| spelling | oai:generic.eprints.org:368412018-06-20T01:15:34Z http://irep.iium.edu.my/36841/ The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo Khaleel, Ruwaidah F. Selman, Mohammad O. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. QM Human anatomy RB Pathology RM300 Drugs and their action Background: Antiepileptic drug (phenytoin) is extensively used by pregnant women who have seizure disorders. It is reported to produce a variety of facial defects, and considered as an important cause of nongenetic cleft palate in their offspring. Objective: To investigate the influence of administrating phenytoin to pregnant rats on the process of palatal fusion in rat embryos. Material & Method: Female pregnant rats (Rattus norvegicus) were divided into two groups: The control group (G1) and experimental group (G2). Each group consist of (30) pregnant rats. Group 2 are subdivided into two subgroups (G2A), (G2B), each group contain 15 pregnant female rats. Each rat was given a dose of 15 mg daily phenytoin was suspended in distilled water to obtain a concentration of 15mg/kg/day , and 0.1 mg/ kg that contain 3mg/rat/day was given I/P. From (E19-E16) of gestation when mothers of G2A reach (E20) of gestation cesarean section done and weighted embryos and cut the head of embryos, however when mothers of G2B reach (E21) of gestation normal delivery occur and weighted embryos and cut the head of embryos of gestation and 50 embryo from each group was used for histological preparation. Result: At embryonic day 20 (E20) and day 21 (E21), there was delay union of palatal shelves, cartilaginous structure persist and was longer Phenytoin treated group than normals. Conclusion: The present study did not demonstrate clefting of the palate in the phenytoin treated group. However, the histological study of the embryonic development of the palate and its morphogenesis has demonstrated that progression of fusion is taking place but with a delay in the midline seam disappearance. Department of Anatomy, National University of Singapore 2014-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36841/1/Influence_of_Ante-Natal_Phenytoin_Therapy.pdf Khaleel, Ruwaidah F. and Selman, Mohammad O. and Al-Ani, Imad Matloub Dally and Al-Salihi, Anam R. (2014) The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo. Annals of Microscopy, 14. pp. 57-63. ISSN 0219-2209 http://www.microscopy.org.sg/journal.htm |
| spellingShingle | QM Human anatomy RB Pathology RM300 Drugs and their action Khaleel, Ruwaidah F. Selman, Mohammad O. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo |
| title | The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo |
| title_full | The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo |
| title_fullStr | The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo |
| title_full_unstemmed | The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo |
| title_short | The influence of ante-natal phenytoin therapy on palatal fusion in rat embryo |
| title_sort | influence of ante natal phenytoin therapy on palatal fusion in rat embryo |
| topic | QM Human anatomy RB Pathology RM300 Drugs and their action |
| url | http://irep.iium.edu.my/36841/1/Influence_of_Ante-Natal_Phenytoin_Therapy.pdf |
| work_keys_str_mv | AT khaleelruwaidahf theinfluenceofantenatalphenytointherapyonpalatalfusioninratembryo AT selmanmohammado theinfluenceofantenatalphenytointherapyonpalatalfusioninratembryo AT alaniimadmatloubdally theinfluenceofantenatalphenytointherapyonpalatalfusioninratembryo AT alsalihianamr theinfluenceofantenatalphenytointherapyonpalatalfusioninratembryo AT khaleelruwaidahf influenceofantenatalphenytointherapyonpalatalfusioninratembryo AT selmanmohammado influenceofantenatalphenytointherapyonpalatalfusioninratembryo AT alaniimadmatloubdally influenceofantenatalphenytointherapyonpalatalfusioninratembryo AT alsalihianamr influenceofantenatalphenytointherapyonpalatalfusioninratembryo |