Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells
Electric cell-substrate impedance sensing (ECIS) method can be used as a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. Changes in impedance of the cells due to growth and attachment can be modeled as an equivalent circuit consisting of resistors an...
| Main Authors: | , , , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/3733/1/30.pdf |
| _version_ | 1825644460221923328 |
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| author | Siddiquei, H. R. Nordin, Anis Nurashikin Ibrahimy, Muhammad Ibn Arifin, Mohd Azmir Sulong, Nurul Hafizah Mel, Maizirwan Voiculescu, Ioana |
| author_facet | Siddiquei, H. R. Nordin, Anis Nurashikin Ibrahimy, Muhammad Ibn Arifin, Mohd Azmir Sulong, Nurul Hafizah Mel, Maizirwan Voiculescu, Ioana |
| author_sort | Siddiquei, H. R. |
| collection | IIUM |
| description | Electric cell-substrate impedance sensing (ECIS) method can be used as a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. Changes in impedance of the cells due to growth and attachment can be modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. In this work, a biosensor which measures the impedance in DF-1 cells (derived from chicken embroyonic fibroblasts and CEF cells) are presented. The biosensor consists of a Teflon cell holder and two gold electrodes. Experimental measurements were conducted using DF-1 cells cultured in DMEM media. Two different experiments were conducted namely; the control experiment (holder contains only DMEM media) and the cell experiment (holder contains both DMEM media and cells). The biosensor was placed in an incubator with optimum settings for cell growth. Impedance measurements were sampled at six hour intervals. Based on these measurements the resistance and capacitance change due to the growth of the DF-1 cells were calculated. It was observed that significant change in resistance and capacitance values occurs in the first six hours, where cell growth and attachment is most active. After this period of time, the cells become confluent and capacitance values become saturated. |
| first_indexed | 2024-03-05T22:33:08Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:3733 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:33:08Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:37332011-11-13T08:23:43Z http://irep.iium.edu.my/3733/ Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells Siddiquei, H. R. Nordin, Anis Nurashikin Ibrahimy, Muhammad Ibn Arifin, Mohd Azmir Sulong, Nurul Hafizah Mel, Maizirwan Voiculescu, Ioana TK7885 Computer engineering Electric cell-substrate impedance sensing (ECIS) method can be used as a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. Changes in impedance of the cells due to growth and attachment can be modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. In this work, a biosensor which measures the impedance in DF-1 cells (derived from chicken embroyonic fibroblasts and CEF cells) are presented. The biosensor consists of a Teflon cell holder and two gold electrodes. Experimental measurements were conducted using DF-1 cells cultured in DMEM media. Two different experiments were conducted namely; the control experiment (holder contains only DMEM media) and the cell experiment (holder contains both DMEM media and cells). The biosensor was placed in an incubator with optimum settings for cell growth. Impedance measurements were sampled at six hour intervals. Based on these measurements the resistance and capacitance change due to the growth of the DF-1 cells were calculated. It was observed that significant change in resistance and capacitance values occurs in the first six hours, where cell growth and attachment is most active. After this period of time, the cells become confluent and capacitance values become saturated. 2010-05 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/3733/1/30.pdf Siddiquei, H. R. and Nordin, Anis Nurashikin and Ibrahimy, Muhammad Ibn and Arifin, Mohd Azmir and Sulong, Nurul Hafizah and Mel, Maizirwan and Voiculescu, Ioana (2010) Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells. In: International Conference on Computer and Communication Engineering (ICCCE 2010), 11-13 May 2010, Kuala Lumpur. http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=5556772&tag=1 |
| spellingShingle | TK7885 Computer engineering Siddiquei, H. R. Nordin, Anis Nurashikin Ibrahimy, Muhammad Ibn Arifin, Mohd Azmir Sulong, Nurul Hafizah Mel, Maizirwan Voiculescu, Ioana Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells |
| title | Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells |
| title_full | Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells |
| title_fullStr | Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells |
| title_full_unstemmed | Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells |
| title_short | Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells |
| title_sort | electrical cell substrate impedance sensing ecis based biosensor for characterization of df 1 cells |
| topic | TK7885 Computer engineering |
| url | http://irep.iium.edu.my/3733/1/30.pdf |
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