| Summary: | pBUC114 is a random amplified polymorphic DNA (RAPD) primer amplified a DNA band in 1060 bp linked to powdery mildew resistence gene (Pm-W) in PMAR5, a powdery mildew resistent melon cultivar. In this study, the RAPD primer was used for Polymerase Chain Reaction (PCR)-RAPD to detect Pm-W gene in 16 melon cultivars. Results of PCR-RAPD showed that among 16 melon cultivars, 4 cultivars (WMR29, Harukei, Action 434 and Nigeashi) did not obtained a DNA band in 1060 bp linked to Pm-W gene and they are suspectible. Whereas, 12 cultivars (PMAR5, PMR45, PMR5, Action 434, PI 414723, PI 124112, PI 124111, PI 371795, Sunrise, MR1, Nigeashi-2, PI 161375 and Kohimeuri) obtaine a DNA band in 1060 bp linked to Pm-W gene and they are resistant. The result of this study revealed that RAPD markers can be used as a tool for rapid screening of resistent melons against powdery mildew.
Key words: melon, Pm-W, powdery mildew, RAPD
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