Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons
pBUC114 is a random amplified polymorphic DNA (RAPD) primer amplified a DNA band in 1060 bp linked to powdery mildew resistence gene (Pm-W) in PMAR5, a powdery mildew resistent melon cultivar. In this study, the RAPD primer was used for Polymerase Chain Reaction (PCR)-RAPD to detect Pm-W gene in 16...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2009
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| Online Access: | https://repository.ugm.ac.id/37677/1/Proceeding%20ICBS%202009.pdf |
| _version_ | 1826033590542008320 |
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| author | Daryono, Budi Setiadi |
| author_facet | Daryono, Budi Setiadi |
| author_sort | Daryono, Budi Setiadi |
| collection | UGM |
| description | pBUC114 is a random amplified polymorphic DNA (RAPD) primer amplified a DNA band in 1060 bp linked to powdery mildew resistence gene (Pm-W) in PMAR5, a powdery mildew resistent melon cultivar. In this study, the RAPD primer was used for Polymerase Chain Reaction (PCR)-RAPD to detect Pm-W gene in 16 melon cultivars. Results of PCR-RAPD showed that among 16 melon cultivars, 4 cultivars (WMR29, Harukei, Action 434 and Nigeashi) did not obtained a DNA band in 1060 bp linked to Pm-W gene and they are suspectible. Whereas, 12 cultivars (PMAR5, PMR45, PMR5, Action 434, PI 414723, PI 124112, PI 124111, PI 371795, Sunrise, MR1, Nigeashi-2, PI 161375 and Kohimeuri) obtaine a DNA band in 1060 bp linked to Pm-W gene and they are resistant. The result of this study revealed that RAPD markers can be used as a tool for rapid screening of resistent melons against powdery mildew.
Key words: melon, Pm-W, powdery mildew, RAPD |
| first_indexed | 2024-03-05T23:28:15Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:37677 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-05T23:28:15Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:376772014-07-18T06:39:55Z https://repository.ugm.ac.id/37677/ Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons Daryono, Budi Setiadi Agricultural Land Management pBUC114 is a random amplified polymorphic DNA (RAPD) primer amplified a DNA band in 1060 bp linked to powdery mildew resistence gene (Pm-W) in PMAR5, a powdery mildew resistent melon cultivar. In this study, the RAPD primer was used for Polymerase Chain Reaction (PCR)-RAPD to detect Pm-W gene in 16 melon cultivars. Results of PCR-RAPD showed that among 16 melon cultivars, 4 cultivars (WMR29, Harukei, Action 434 and Nigeashi) did not obtained a DNA band in 1060 bp linked to Pm-W gene and they are suspectible. Whereas, 12 cultivars (PMAR5, PMR45, PMR5, Action 434, PI 414723, PI 124112, PI 124111, PI 371795, Sunrise, MR1, Nigeashi-2, PI 161375 and Kohimeuri) obtaine a DNA band in 1060 bp linked to Pm-W gene and they are resistant. The result of this study revealed that RAPD markers can be used as a tool for rapid screening of resistent melons against powdery mildew. Key words: melon, Pm-W, powdery mildew, RAPD 2009-10-17 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/37677/1/Proceeding%20ICBS%202009.pdf Daryono, Budi Setiadi (2009) Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons. In: International Conference on Biological Science, 16-17 Oktober, 2009, Yogyakarta, Indonesia. |
| spellingShingle | Agricultural Land Management Daryono, Budi Setiadi Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons |
| title | Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons |
| title_full | Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons |
| title_fullStr | Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons |
| title_full_unstemmed | Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons |
| title_short | Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons |
| title_sort | application of random amplified polimorphic dna rapd markers for detection of powdery mildew resistence gene in diverse melons |
| topic | Agricultural Land Management |
| url | https://repository.ugm.ac.id/37677/1/Proceeding%20ICBS%202009.pdf |
| work_keys_str_mv | AT daryonobudisetiadi applicationofrandomamplifiedpolimorphicdnarapdmarkersfordetectionofpowderymildewresistencegeneindiversemelons |