| Summary: | Wonosadi forest is located between Dusun Duren and Dusun Sidorejo, Beji village,
Ngawen, Gunungkidul. The biodiversity inside Wonosadi is protected by people around Wonosadi
using local wisdom. One of the endemic biological diversity in Wonosadi is natural orchid.
Conservation which is related to biodiversity were needed to maintain the existence of natural orchid
in Wonosadi sustainly. Study on natural orchid in Wonosadi can be used as the database for
conservation programs. In this study, genetic variation was analysed usi ng random amplified
polymorphic DNA (RAPD), while viruses were detected using reverse transcript-polymerase chain
reaction (RT-PCR).
The results show that there were genetic diversity in the populations of natural orchid in
Wonosadi. It can be concluded that the population of natural orchid in Wonosadi can be adaptive to
environmental change. Genetic diversity is required for populations to evolve to cope with
environmental change. It can be used as a database to develop the potential of natural orchid in
Wonosadi forest. The viruses found in population of natural orchid in Wonosadi were Cymbidium
mosaic virus (CyMV) and Odontoglossum ringspot virus (ORSV). Viruses existance might be
recognised from the physical symptom on plant and analysis of coat protein (CP) gene using
polymerase chain reaction (PCR).
The research of diversity and conservation of natural orchid in Wonosadi forest ma y be used
to support education for sustainable development (EfSD) concept in conservation of biological
diversity. Exploration activity can be focused at area which is protected by local wisdom and involves
local people. By doing this, local people may be actively included on protecting and developing
biological diversity at their own comunity. Thus, it may result a sustain condition of natural resources.
This research can be used in supporting and developing programs of natural orchid in Wonosadi
forest in order to develop the conservation programs.
Keywords : genetic diversity, natural orchid, CyMV, ORSV, conservation
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