THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON

The inheritance of resistance to an Indonesian isolate of powdery mildew in melon cultivar PI 371795 was previously determined and it is controlled by a single dominant gene, to which the symbol Pm-W was assigned. However, the origin of Pm-W gene in PI 371795 and their generations were not character...

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Bibliographic Details
Main Authors: Kasiamdari, Rina Sri, Aristya, Ganies Riza, Daryono, Budi Setiadi
Format: Conference or Workshop Item
Language:English
Published: 2009
Subjects:
Online Access:https://repository.ugm.ac.id/37692/1/Proceeding%20ICBS%202009_Rina.pdf
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Summary:The inheritance of resistance to an Indonesian isolate of powdery mildew in melon cultivar PI 371795 was previously determined and it is controlled by a single dominant gene, to which the symbol Pm-W was assigned. However, the origin of Pm-W gene in PI 371795 and their generations were not characterized. In this study we sought to define the inheritance of Pm-Wgene among resistant cultivars PI 371795, susceptible cultivar Action 434, F1, F2, and Testcross generations by RAPD marker analysis. As the result, a RAPD marker which produced the specific DNA bands linked to PmW was shown only in resistant cultivar of PI 371795, F1 generation, F2 and Testcross resistant. However, the DNA band was not shown in the susceptible cultivars Action 434, F2 and Testcross susceptible, respectively. This result revealed that a specific DNA band linked to Pm-Wgene in melon PI 371795 and their generations was derived from the resistant parental cultivar of PI 371795 Keywords: Melon, powdery mildew, resistance gene, RAPD