THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON
The inheritance of resistance to an Indonesian isolate of powdery mildew in melon cultivar PI 371795 was previously determined and it is controlled by a single dominant gene, to which the symbol Pm-W was assigned. However, the origin of Pm-W gene in PI 371795 and their generations were not character...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2009
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| Online Access: | https://repository.ugm.ac.id/37692/1/Proceeding%20ICBS%202009_Rina.pdf |
| _version_ | 1826033592084463616 |
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| author | Kasiamdari, Rina Sri Aristya, Ganies Riza Daryono, Budi Setiadi |
| author_facet | Kasiamdari, Rina Sri Aristya, Ganies Riza Daryono, Budi Setiadi |
| author_sort | Kasiamdari, Rina Sri |
| collection | UGM |
| description | The inheritance of resistance to an Indonesian isolate of powdery mildew in melon cultivar PI 371795 was previously determined and it is controlled by a single dominant gene, to which the symbol Pm-W was assigned. However, the origin of Pm-W gene in PI 371795 and their generations were not characterized. In this study we sought to define the inheritance of Pm-Wgene among resistant cultivars PI 371795, susceptible cultivar Action 434, F1, F2, and Testcross generations by RAPD marker analysis. As the result, a RAPD marker which produced the specific DNA bands linked to PmW was shown only in resistant cultivar of PI 371795, F1 generation, F2 and Testcross resistant. However, the DNA band was not shown in the susceptible cultivars Action 434, F2 and Testcross susceptible, respectively. This result revealed that a specific DNA band linked to Pm-Wgene in melon PI 371795 and their generations was derived from the resistant parental cultivar of PI 371795
Keywords: Melon, powdery mildew, resistance gene, RAPD |
| first_indexed | 2024-03-13T19:14:10Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:37692 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T19:14:10Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:376922014-07-21T04:08:24Z https://repository.ugm.ac.id/37692/ THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON Kasiamdari, Rina Sri Aristya, Ganies Riza Daryono, Budi Setiadi Agricultural Land Management The inheritance of resistance to an Indonesian isolate of powdery mildew in melon cultivar PI 371795 was previously determined and it is controlled by a single dominant gene, to which the symbol Pm-W was assigned. However, the origin of Pm-W gene in PI 371795 and their generations were not characterized. In this study we sought to define the inheritance of Pm-Wgene among resistant cultivars PI 371795, susceptible cultivar Action 434, F1, F2, and Testcross generations by RAPD marker analysis. As the result, a RAPD marker which produced the specific DNA bands linked to PmW was shown only in resistant cultivar of PI 371795, F1 generation, F2 and Testcross resistant. However, the DNA band was not shown in the susceptible cultivars Action 434, F2 and Testcross susceptible, respectively. This result revealed that a specific DNA band linked to Pm-Wgene in melon PI 371795 and their generations was derived from the resistant parental cultivar of PI 371795 Keywords: Melon, powdery mildew, resistance gene, RAPD 2009-10-17 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/37692/1/Proceeding%20ICBS%202009_Rina.pdf Kasiamdari, Rina Sri and Aristya, Ganies Riza and Daryono, Budi Setiadi (2009) THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON. In: International Conference On Biological Science, 16-17 Oktober, 2009, Yogyakarta, Indonesia. |
| spellingShingle | Agricultural Land Management Kasiamdari, Rina Sri Aristya, Ganies Riza Daryono, Budi Setiadi THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON |
| title | THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON |
| title_full | THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON |
| title_fullStr | THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON |
| title_full_unstemmed | THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON |
| title_short | THE INHERITANCE OF RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD) MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE IN MELON |
| title_sort | inheritance of random amplified polimorphic dna rapd markers linked to powdery mildew resistance gene in melon |
| topic | Agricultural Land Management |
| url | https://repository.ugm.ac.id/37692/1/Proceeding%20ICBS%202009_Rina.pdf |
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