Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon
Creb-2, a gne resistance to an Indonesian isolate of cucumber mosaic virus (CMV_B2) in melon cultivar ‘Yamatouri’ has previously been described as single dominant gene. Eleven melon resistance gene analogs (MRGAs) were initially isolated to elucidate the resistance mechanism when used as a probe for...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2009
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| Online Access: | https://repository.ugm.ac.id/37706/1/7.pdf |
| _version_ | 1826033593427689472 |
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| author | Daryono, Budi Setiadi |
| author_facet | Daryono, Budi Setiadi |
| author_sort | Daryono, Budi Setiadi |
| collection | UGM |
| description | Creb-2, a gne resistance to an Indonesian isolate of cucumber mosaic virus (CMV_B2) in melon cultivar ‘Yamatouri’ has previously been described as single dominant gene. Eleven melon resistance gene analogs (MRGAs) were initially isolated to elucidate the resistance mechanism when used as a probe for restriction fragment length polymorphism (RFLP) analysis against ‘Yamatouri’ and ‘Vakharman’ for the Creb-2. MRGA1 was then developed and two set of sequence-tagged site (STS) primers (MRGA-11 and MRGA-21) were designed. However, only MRGA-11 primers produced a polymorphism. The MRGA-11 was applied for linkage map of Creb-2 in 104 F2 progeny. Linkage analysis showed that genetic distance of MRGA-11 to the Creb-2 was 0.96 cM. MRGA-11 marker could be used for screening of CMV-B2 resistant cultivar in F2 populations and makuwa melons. |
| first_indexed | 2024-03-05T23:28:18Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:37706 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-05T23:28:18Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:377062014-07-24T01:01:26Z https://repository.ugm.ac.id/37706/ Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon Daryono, Budi Setiadi Agricultural Land Management Creb-2, a gne resistance to an Indonesian isolate of cucumber mosaic virus (CMV_B2) in melon cultivar ‘Yamatouri’ has previously been described as single dominant gene. Eleven melon resistance gene analogs (MRGAs) were initially isolated to elucidate the resistance mechanism when used as a probe for restriction fragment length polymorphism (RFLP) analysis against ‘Yamatouri’ and ‘Vakharman’ for the Creb-2. MRGA1 was then developed and two set of sequence-tagged site (STS) primers (MRGA-11 and MRGA-21) were designed. However, only MRGA-11 primers produced a polymorphism. The MRGA-11 was applied for linkage map of Creb-2 in 104 F2 progeny. Linkage analysis showed that genetic distance of MRGA-11 to the Creb-2 was 0.96 cM. MRGA-11 marker could be used for screening of CMV-B2 resistant cultivar in F2 populations and makuwa melons. 2009-09-22 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/37706/1/7.pdf Daryono, Budi Setiadi (2009) Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon. In: 4th International Symposium on Cucurbits, 21-26 September 2009, Changsha, Hunan, China. |
| spellingShingle | Agricultural Land Management Daryono, Budi Setiadi Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon |
| title | Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon |
| title_full | Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon |
| title_fullStr | Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon |
| title_full_unstemmed | Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon |
| title_short | Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon |
| title_sort | development and application of melon resistance gene analog mrga markers for detection of virus resistance gene in melon |
| topic | Agricultural Land Management |
| url | https://repository.ugm.ac.id/37706/1/7.pdf |
| work_keys_str_mv | AT daryonobudisetiadi developmentandapplicationofmelonresistancegeneanalogmrgamarkersfordetectionofvirusresistancegeneinmelon |