Bayesian prokaryote classification from microscopic images

Great knowledge and experience on microbiology are required for accurate bacteria identification. Automation of bacteria identification is required because there might be a shortage of skilled microbiologists and clinicians at a time of great need. We propose an automatic bacteria identification fr...

Full description

Bibliographic Details
Main Authors: Mohamad, Noor Amaleena, Jusoh Awang, Noorain, Htike@Muhammad Yusof, Zaw Zaw, Shoon , Lei Win
Format: Article
Language:English
Published: AIRCC Publishing Corporation 2014
Subjects:
Online Access:http://irep.iium.edu.my/38132/1/1114caij01.pdf
_version_ 1796878985438691328
author Mohamad, Noor Amaleena
Jusoh Awang, Noorain
Htike@Muhammad Yusof, Zaw Zaw
Shoon , Lei Win
author_facet Mohamad, Noor Amaleena
Jusoh Awang, Noorain
Htike@Muhammad Yusof, Zaw Zaw
Shoon , Lei Win
author_sort Mohamad, Noor Amaleena
collection IIUM
description Great knowledge and experience on microbiology are required for accurate bacteria identification. Automation of bacteria identification is required because there might be a shortage of skilled microbiologists and clinicians at a time of great need. We propose an automatic bacteria identification framework that can classify three famous classes of bacteria namely Cocci, Bacilli and Vibrio from microscopic morphology using the Naïve Bayes classifier. The proposed bacteria identification framework comprises two steps. In the first step, the system is trained using a set of microscopic images containing Cocci, Bacilli, and Vibrio. The input images are normalized to emphasize the diameter and shape features. Edge-based descriptors are then extracted from the input images. In the second step, we use the Naïve Bayes classifier to perform probabilistic inference based on the input descriptors. 64 images for each class of bacteria were used as the training set and 222 images consisting of the three classes of bacteria and other random images such as humans and airplanes were used as the test set. There are no images overlapped between the training set and the test set. The system was found to be able to accurately discriminate the three classes of bacteria. Moreover, the system was also found to be able to reject images that did not belong to any of the three classes of bacteria. The preliminary results demonstrate how a simple machine learning classifier with a set of simple image-based features can result in high classification accuracy. The preliminary results also demonstrate the efficacy and efficiency of our two-step automatic bacteria identification approach and motivate us to extend this framework to identify a variety of other types of bacteria.
first_indexed 2024-03-05T23:29:09Z
format Article
id oai:generic.eprints.org:38132
institution International Islamic University Malaysia
language English
last_indexed 2024-03-05T23:29:09Z
publishDate 2014
publisher AIRCC Publishing Corporation
record_format dspace
spelling oai:generic.eprints.org:381322018-06-19T04:14:28Z http://irep.iium.edu.my/38132/ Bayesian prokaryote classification from microscopic images Mohamad, Noor Amaleena Jusoh Awang, Noorain Htike@Muhammad Yusof, Zaw Zaw Shoon , Lei Win Q Science (General) Great knowledge and experience on microbiology are required for accurate bacteria identification. Automation of bacteria identification is required because there might be a shortage of skilled microbiologists and clinicians at a time of great need. We propose an automatic bacteria identification framework that can classify three famous classes of bacteria namely Cocci, Bacilli and Vibrio from microscopic morphology using the Naïve Bayes classifier. The proposed bacteria identification framework comprises two steps. In the first step, the system is trained using a set of microscopic images containing Cocci, Bacilli, and Vibrio. The input images are normalized to emphasize the diameter and shape features. Edge-based descriptors are then extracted from the input images. In the second step, we use the Naïve Bayes classifier to perform probabilistic inference based on the input descriptors. 64 images for each class of bacteria were used as the training set and 222 images consisting of the three classes of bacteria and other random images such as humans and airplanes were used as the test set. There are no images overlapped between the training set and the test set. The system was found to be able to accurately discriminate the three classes of bacteria. Moreover, the system was also found to be able to reject images that did not belong to any of the three classes of bacteria. The preliminary results demonstrate how a simple machine learning classifier with a set of simple image-based features can result in high classification accuracy. The preliminary results also demonstrate the efficacy and efficiency of our two-step automatic bacteria identification approach and motivate us to extend this framework to identify a variety of other types of bacteria. AIRCC Publishing Corporation 2014-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38132/1/1114caij01.pdf Mohamad, Noor Amaleena and Jusoh Awang, Noorain and Htike@Muhammad Yusof, Zaw Zaw and Shoon , Lei Win (2014) Bayesian prokaryote classification from microscopic images. Computer Applications: An International Journal (CAIJ), 1 (1). pp. 1-9. ISSN 2393-8455 http://airccse.com/caij/current.html
spellingShingle Q Science (General)
Mohamad, Noor Amaleena
Jusoh Awang, Noorain
Htike@Muhammad Yusof, Zaw Zaw
Shoon , Lei Win
Bayesian prokaryote classification from microscopic images
title Bayesian prokaryote classification from microscopic images
title_full Bayesian prokaryote classification from microscopic images
title_fullStr Bayesian prokaryote classification from microscopic images
title_full_unstemmed Bayesian prokaryote classification from microscopic images
title_short Bayesian prokaryote classification from microscopic images
title_sort bayesian prokaryote classification from microscopic images
topic Q Science (General)
url http://irep.iium.edu.my/38132/1/1114caij01.pdf
work_keys_str_mv AT mohamadnooramaleena bayesianprokaryoteclassificationfrommicroscopicimages
AT jusohawangnoorain bayesianprokaryoteclassificationfrommicroscopicimages
AT htikemuhammadyusofzawzaw bayesianprokaryoteclassificationfrommicroscopicimages
AT shoonleiwin bayesianprokaryoteclassificationfrommicroscopicimages