Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction
Horse mango (Mangifera foetida Lour) fruit is known to produce latex with a blistering effect upon contact with human skin. In this study, it was chosen as a source of protease, and the effect of the extraction process on its protease activity was evaluated. The crude enzyme was extracted from the k...
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| Format: | Article |
| Language: | English |
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Malaysia Institute of Chemistry
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/38466/1/Malaysian_Journal_of_Chemistry%2C_2013%2C_Vol._15%2C_No._1%2C_020_%E2%80%93_026.pdf |
| _version_ | 1796879041558478848 |
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| author | Ahmad, Mohammad Norazmi Ibrahim, Izyanti Yarmo, Mohd Ambar Said, Mamot |
| author_facet | Ahmad, Mohammad Norazmi Ibrahim, Izyanti Yarmo, Mohd Ambar Said, Mamot |
| author_sort | Ahmad, Mohammad Norazmi |
| collection | IIUM |
| description | Horse mango (Mangifera foetida Lour) fruit is known to produce latex with a blistering effect upon contact with human skin. In this study, it was chosen as a source of protease, and the effect of the extraction process on its protease activity was evaluated. The crude enzyme was extracted from the kernels and extraction was optimized by a response surface methodology (RSM) using a central composite rotatable design (CCRD). Based on the RSM model generated, optimal extraction conditions were obtained at pH 6.0, 8.16 mM CaCl2, 5.0% Triton X-100, and 10.0 mM DTT. Polyvinylpolypyrrolidone (PVPP) and Dowex 2-X8 were added to the medium extraction to remove the polyphenols from the crude extract. The result indicated that, the media which was supplemented with the polyphenols binders acquired the high purified protease compared to the control (media without PVPP and Dowex 2-X8). |
| first_indexed | 2024-03-05T23:29:52Z |
| format | Article |
| id | oai:generic.eprints.org:38466 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:29:52Z |
| publishDate | 2013 |
| publisher | Malaysia Institute of Chemistry |
| record_format | dspace |
| spelling | oai:generic.eprints.org:384662016-10-20T04:21:18Z http://irep.iium.edu.my/38466/ Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction Ahmad, Mohammad Norazmi Ibrahim, Izyanti Yarmo, Mohd Ambar Said, Mamot QD Chemistry Horse mango (Mangifera foetida Lour) fruit is known to produce latex with a blistering effect upon contact with human skin. In this study, it was chosen as a source of protease, and the effect of the extraction process on its protease activity was evaluated. The crude enzyme was extracted from the kernels and extraction was optimized by a response surface methodology (RSM) using a central composite rotatable design (CCRD). Based on the RSM model generated, optimal extraction conditions were obtained at pH 6.0, 8.16 mM CaCl2, 5.0% Triton X-100, and 10.0 mM DTT. Polyvinylpolypyrrolidone (PVPP) and Dowex 2-X8 were added to the medium extraction to remove the polyphenols from the crude extract. The result indicated that, the media which was supplemented with the polyphenols binders acquired the high purified protease compared to the control (media without PVPP and Dowex 2-X8). Malaysia Institute of Chemistry 2013-03-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38466/1/Malaysian_Journal_of_Chemistry%2C_2013%2C_Vol._15%2C_No._1%2C_020_%E2%80%93_026.pdf Ahmad, Mohammad Norazmi and Ibrahim, Izyanti and Yarmo, Mohd Ambar and Said, Mamot (2013) Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction. Protease of the Horse Mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction, 15 (1). pp. 20-26. ISSN 1511-2292 http://www.ikm.org.my/index.php?option=com_content&view=article&id=225&Itemid=389 |
| spellingShingle | QD Chemistry Ahmad, Mohammad Norazmi Ibrahim, Izyanti Yarmo, Mohd Ambar Said, Mamot Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction |
| title | Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction |
| title_full | Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction |
| title_fullStr | Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction |
| title_full_unstemmed | Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction |
| title_short | Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction |
| title_sort | protease of the horse mango mangifera foetida lour kernel optimum conditions for extraction |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/38466/1/Malaysian_Journal_of_Chemistry%2C_2013%2C_Vol._15%2C_No._1%2C_020_%E2%80%93_026.pdf |
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