Dynamics behavior of Endoglucanase from Fusarium oxysporum
Molecular dynamics methods are very useful tool in understanding the behavior of the enzymes at higher temperatures. It is well known that some enzymes lose their enzyme activity at higher temperatures. In this work we employ molecular dynamics simulation of an endoglucanase from Fusarium oxyspor...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/3911/1/Dyn_Behavr_of_EG_from_F_oxysporum_pg286-288.pdf |
| _version_ | 1825644479662522368 |
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| author | Noorbatcha, Ibrahim Ali S., Waesoho Mohd. Salleh, Hamzah |
| author_facet | Noorbatcha, Ibrahim Ali S., Waesoho Mohd. Salleh, Hamzah |
| author_sort | Noorbatcha, Ibrahim Ali |
| collection | IIUM |
| description | Molecular dynamics methods are very useful tool in
understanding the behavior of the enzymes at higher
temperatures. It is well known that some enzymes lose their
enzyme activity at higher temperatures. In this work we employ molecular dynamics simulation of an endoglucanase from Fusarium oxysporum (EGFO) to understand the reasons behind the loss of enzyme activity when the temperatures is increased form 40°C to 80°C. The dynamic behavior of this enzyme is analyzed by examining the Root Mean Square Derivation(RMSD), salt bridges, gyration ratio and hydrogen bonds at different temperatures as a function of time. It has been found that there are differences in (i) the RMSD of backbone and coil regions, (ii) the number of hydrogen bonds among amino acidresidues and between amino acid residues and explicit watermolecules within the coil region of the enzyme (iii) total number of salt bridges, and (iv) the gyration ratio of the enzyme at different temperatures. These factors could play significant role in the loss of activity of EGFO as the temperature is increased. |
| first_indexed | 2024-03-05T22:33:25Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:3911 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:33:25Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:39112021-06-29T02:35:21Z http://irep.iium.edu.my/3911/ Dynamics behavior of Endoglucanase from Fusarium oxysporum Noorbatcha, Ibrahim Ali S., Waesoho Mohd. Salleh, Hamzah TP248.13 Biotechnology Molecular dynamics methods are very useful tool in understanding the behavior of the enzymes at higher temperatures. It is well known that some enzymes lose their enzyme activity at higher temperatures. In this work we employ molecular dynamics simulation of an endoglucanase from Fusarium oxysporum (EGFO) to understand the reasons behind the loss of enzyme activity when the temperatures is increased form 40°C to 80°C. The dynamic behavior of this enzyme is analyzed by examining the Root Mean Square Derivation(RMSD), salt bridges, gyration ratio and hydrogen bonds at different temperatures as a function of time. It has been found that there are differences in (i) the RMSD of backbone and coil regions, (ii) the number of hydrogen bonds among amino acidresidues and between amino acid residues and explicit watermolecules within the coil region of the enzyme (iii) total number of salt bridges, and (iv) the gyration ratio of the enzyme at different temperatures. These factors could play significant role in the loss of activity of EGFO as the temperature is increased. 2011-05-17 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/3911/1/Dyn_Behavr_of_EG_from_F_oxysporum_pg286-288.pdf Noorbatcha, Ibrahim Ali and S., Waesoho and Mohd. Salleh, Hamzah (2011) Dynamics behavior of Endoglucanase from Fusarium oxysporum. In: International Conference on Biotechnology Engineering, ICBioE’11, 17-19 May, 2011 , Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | TP248.13 Biotechnology Noorbatcha, Ibrahim Ali S., Waesoho Mohd. Salleh, Hamzah Dynamics behavior of Endoglucanase from Fusarium oxysporum |
| title | Dynamics behavior of Endoglucanase from Fusarium
oxysporum |
| title_full | Dynamics behavior of Endoglucanase from Fusarium
oxysporum |
| title_fullStr | Dynamics behavior of Endoglucanase from Fusarium
oxysporum |
| title_full_unstemmed | Dynamics behavior of Endoglucanase from Fusarium
oxysporum |
| title_short | Dynamics behavior of Endoglucanase from Fusarium
oxysporum |
| title_sort | dynamics behavior of endoglucanase from fusarium oxysporum |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/3911/1/Dyn_Behavr_of_EG_from_F_oxysporum_pg286-288.pdf |
| work_keys_str_mv | AT noorbatchaibrahimali dynamicsbehaviorofendoglucanasefromfusariumoxysporum AT swaesoho dynamicsbehaviorofendoglucanasefromfusariumoxysporum AT mohdsallehhamzah dynamicsbehaviorofendoglucanasefromfusariumoxysporum |