Improvement of phytase properties by rational design intended for animal feed
For application in animal feed, a phytase of interest must have high activity in the broad range prevalent industrial condition and in digestive tract of the animal. The present investigation describes approaches to rationally engineer a phytase with better activity profiles. Computer modelling is...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/4019/1/IRIIE_2010_Abstract_Phytase_P201.pdf |
| _version_ | 1825644494270234624 |
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| author | Samsudin, Nurhusna Noorbatcha, Ibrahim Ali Amid, Azura Mohd. Salleh, Hamzah |
| author_facet | Samsudin, Nurhusna Noorbatcha, Ibrahim Ali Amid, Azura Mohd. Salleh, Hamzah |
| author_sort | Samsudin, Nurhusna |
| collection | IIUM |
| description | For application in animal feed, a phytase of interest must have high activity in the broad range
prevalent industrial condition and in digestive tract of the animal. The present investigation describes
approaches to rationally engineer a phytase with better activity profiles. Computer modelling is used to
identify and examine active site of phyFAUIA1 phytase. The factors influencing the ligand binding
strength in the active site is analyzed and computational site directed mutagenesis experiments were
carried out to evaluate the effects of mutations on the binding strength before and after mutation. From
the directive results of computational studies, point mutation was introduced by site directed mutagenesis
using polymerase chain reaction (PCR). Mutagenesis is achieved by two steps PCR procedure. Four
primers were designed and synthesized: two primers contain mutation at the point of interest which is
complement to each other and the other two primers were designed for unique restriction sites. Several
numbers of single, double as well as triple mutations have been introduced in phyFAUIA1 gene, and the
mutant enzymes will be characterized and compared to computational results. |
| first_indexed | 2024-03-05T22:33:38Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:4019 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:33:38Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:40192021-06-23T03:29:34Z http://irep.iium.edu.my/4019/ Improvement of phytase properties by rational design intended for animal feed Samsudin, Nurhusna Noorbatcha, Ibrahim Ali Amid, Azura Mohd. Salleh, Hamzah TP248.13 Biotechnology For application in animal feed, a phytase of interest must have high activity in the broad range prevalent industrial condition and in digestive tract of the animal. The present investigation describes approaches to rationally engineer a phytase with better activity profiles. Computer modelling is used to identify and examine active site of phyFAUIA1 phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength before and after mutation. From the directive results of computational studies, point mutation was introduced by site directed mutagenesis using polymerase chain reaction (PCR). Mutagenesis is achieved by two steps PCR procedure. Four primers were designed and synthesized: two primers contain mutation at the point of interest which is complement to each other and the other two primers were designed for unique restriction sites. Several numbers of single, double as well as triple mutations have been introduced in phyFAUIA1 gene, and the mutant enzymes will be characterized and compared to computational results. 2010-01-26 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/4019/1/IRIIE_2010_Abstract_Phytase_P201.pdf Samsudin, Nurhusna and Noorbatcha, Ibrahim Ali and Amid, Azura and Mohd. Salleh, Hamzah (2010) Improvement of phytase properties by rational design intended for animal feed. In: IIUM Research, Innovation & Invention Exhibition, (IRIIE 2010), 26 - 27 January 2010, IIUM. |
| spellingShingle | TP248.13 Biotechnology Samsudin, Nurhusna Noorbatcha, Ibrahim Ali Amid, Azura Mohd. Salleh, Hamzah Improvement of phytase properties by rational design intended for animal feed |
| title | Improvement of phytase properties by rational design
intended for animal feed |
| title_full | Improvement of phytase properties by rational design
intended for animal feed |
| title_fullStr | Improvement of phytase properties by rational design
intended for animal feed |
| title_full_unstemmed | Improvement of phytase properties by rational design
intended for animal feed |
| title_short | Improvement of phytase properties by rational design
intended for animal feed |
| title_sort | improvement of phytase properties by rational design intended for animal feed |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/4019/1/IRIIE_2010_Abstract_Phytase_P201.pdf |
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