Comparison of alcohol free GSH production by ultrasonic and homogenizing method
Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the product...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iranian Journal of Chemistry and Chemical Engineering
2008
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/4108/1/200827414.pdf |
| _version_ | 1825644506427424768 |
|---|---|
| author | Mohamed Salleh, Mohamad Ramlan Jamal, Parveen Gad, Abd. Elaziem Farouk Hashim, Yumi Zuhanis Has-Yun Salleh, Rohani Hasim, Haslinda |
| author_facet | Mohamed Salleh, Mohamad Ramlan Jamal, Parveen Gad, Abd. Elaziem Farouk Hashim, Yumi Zuhanis Has-Yun Salleh, Rohani Hasim, Haslinda |
| author_sort | Mohamed Salleh, Mohamad Ramlan |
| collection | IIUM |
| description | Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the production of the GSH. The cell disruptions were run at a wide range of those parameters at the suitable common parameters of the methods. The disrupted cells suspension was then centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the absorbance (A) value of the solution by spectrophotometer at 412 nm. The results obtained showed that the production of GSH was affected by the adjusted specific parameters of each method and the yield of production by the homogenizing and ultrasonic method was found 3.3 and 4.1 times, respectively, better than the yield of production by the unadjusted parameters. Ultrasonic method which produced 23.2 mg/L product was found 1.3 times better than the homogenizing method (18.5 mg/L) in producing the GSH. The result obtained from the lab scale study of the above mentioned mechanical methods showed that the implementation of them in the large scale production of the alcohol free GSH is possible. |
| first_indexed | 2024-03-05T22:33:49Z |
| format | Article |
| id | oai:generic.eprints.org:4108 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:33:49Z |
| publishDate | 2008 |
| publisher | Iranian Journal of Chemistry and Chemical Engineering |
| record_format | dspace |
| spelling | oai:generic.eprints.org:41082013-07-19T03:00:48Z http://irep.iium.edu.my/4108/ Comparison of alcohol free GSH production by ultrasonic and homogenizing method Mohamed Salleh, Mohamad Ramlan Jamal, Parveen Gad, Abd. Elaziem Farouk Hashim, Yumi Zuhanis Has-Yun Salleh, Rohani Hasim, Haslinda TP248.13 Biotechnology Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the production of the GSH. The cell disruptions were run at a wide range of those parameters at the suitable common parameters of the methods. The disrupted cells suspension was then centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the absorbance (A) value of the solution by spectrophotometer at 412 nm. The results obtained showed that the production of GSH was affected by the adjusted specific parameters of each method and the yield of production by the homogenizing and ultrasonic method was found 3.3 and 4.1 times, respectively, better than the yield of production by the unadjusted parameters. Ultrasonic method which produced 23.2 mg/L product was found 1.3 times better than the homogenizing method (18.5 mg/L) in producing the GSH. The result obtained from the lab scale study of the above mentioned mechanical methods showed that the implementation of them in the large scale production of the alcohol free GSH is possible. Iranian Journal of Chemistry and Chemical Engineering 2008-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4108/1/200827414.pdf Mohamed Salleh, Mohamad Ramlan and Jamal, Parveen and Gad, Abd. Elaziem Farouk and Hashim, Yumi Zuhanis Has-Yun and Salleh, Rohani and Hasim, Haslinda (2008) Comparison of alcohol free GSH production by ultrasonic and homogenizing method. Iranian Journal of Chemistry & Chemical Engineering, 27 (4). pp. 107-113. ISSN 1021-9986 http://www.ijcce.ac.ir/IJCCE/DetailArticleP.asp?Volume=27&Number=4&Ordered=14 |
| spellingShingle | TP248.13 Biotechnology Mohamed Salleh, Mohamad Ramlan Jamal, Parveen Gad, Abd. Elaziem Farouk Hashim, Yumi Zuhanis Has-Yun Salleh, Rohani Hasim, Haslinda Comparison of alcohol free GSH production by ultrasonic and homogenizing method |
| title | Comparison of alcohol free GSH production by ultrasonic and homogenizing method |
| title_full | Comparison of alcohol free GSH production by ultrasonic and homogenizing method |
| title_fullStr | Comparison of alcohol free GSH production by ultrasonic and homogenizing method |
| title_full_unstemmed | Comparison of alcohol free GSH production by ultrasonic and homogenizing method |
| title_short | Comparison of alcohol free GSH production by ultrasonic and homogenizing method |
| title_sort | comparison of alcohol free gsh production by ultrasonic and homogenizing method |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/4108/1/200827414.pdf |
| work_keys_str_mv | AT mohamedsallehmohamadramlan comparisonofalcoholfreegshproductionbyultrasonicandhomogenizingmethod AT jamalparveen comparisonofalcoholfreegshproductionbyultrasonicandhomogenizingmethod AT gadabdelaziemfarouk comparisonofalcoholfreegshproductionbyultrasonicandhomogenizingmethod AT hashimyumizuhanishasyun comparisonofalcoholfreegshproductionbyultrasonicandhomogenizingmethod AT sallehrohani comparisonofalcoholfreegshproductionbyultrasonicandhomogenizingmethod AT hasimhaslinda comparisonofalcoholfreegshproductionbyultrasonicandhomogenizingmethod |