Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds
The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)23-hydroxybutyric acid] (PHB) b...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Wiley
2015
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47514/7/JBMA_35573.pdf http://irep.iium.edu.my/47514/10/47514_Differential%20osteogenic%20potential_WOS_SCOPUS.pdf |
| _version_ | 1825649320381120512 |
|---|---|
| author | Rozila, Ismail Azari, Pedram Munirah, Sha'ban Wan Safwani, Wan Kamarul Zaman Gan, Seng Neon Nur Azurah, Abdul Ghani Jahendran, Jeevanan Pingguan-Murphy, Belinda Chua, Kien Hui |
| author_facet | Rozila, Ismail Azari, Pedram Munirah, Sha'ban Wan Safwani, Wan Kamarul Zaman Gan, Seng Neon Nur Azurah, Abdul Ghani Jahendran, Jeevanan Pingguan-Murphy, Belinda Chua, Kien Hui |
| author_sort | Rozila, Ismail |
| collection | IIUM |
| description | The osteogenic potential of human adipose-derived
stem cells (HADSCs) co-cultured with human osteoblasts
(HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and
1:2, respectively, is evaluated. The HADSCs/HOBs were
seeded on electrospun three-dimensional poly[(R)23-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups. The effects of PHB–BHA scaffold on cell proliferation and cell morphology were assessed by AlamarBlue assay and field emission scanning electron microscopy. Cell differentiation, cell mineralization, and osteogenic related gene expression of co-culture HADSCs/HOBs were examined by alkaline phosphatase (ALP) assay, alizarin Red S assay, and quantitative real time PCR, respectively. The results showed that co-culture of HADSCs/HOBs, 1:1 grown into PHB–BHA promoted better cell adhesion, displayed a significant higher cell proliferation, higher production of ALP, extracellular mineralization and osteogenic-related gene expression of run-related transcription factor, bone sialoprotein, osteopontin, and osteocalcin compared to other coculture groups. This result also suggests that the use of electrospun PHB–BHA in a co-culture HADSCs/HOBs system may serve as promising approach to facilitate osteogenic differentiation
activity of HADSCs through direct cell-to-cell contact
with HOBs. |
| first_indexed | 2024-03-05T23:45:52Z |
| format | Article |
| id | oai:generic.eprints.org:47514 |
| institution | International Islamic University Malaysia |
| language | English English |
| last_indexed | 2024-03-05T23:45:52Z |
| publishDate | 2015 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oai:generic.eprints.org:475142018-05-21T04:14:19Z http://irep.iium.edu.my/47514/ Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds Rozila, Ismail Azari, Pedram Munirah, Sha'ban Wan Safwani, Wan Kamarul Zaman Gan, Seng Neon Nur Azurah, Abdul Ghani Jahendran, Jeevanan Pingguan-Murphy, Belinda Chua, Kien Hui R Medicine (General) The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)23-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups. The effects of PHB–BHA scaffold on cell proliferation and cell morphology were assessed by AlamarBlue assay and field emission scanning electron microscopy. Cell differentiation, cell mineralization, and osteogenic related gene expression of co-culture HADSCs/HOBs were examined by alkaline phosphatase (ALP) assay, alizarin Red S assay, and quantitative real time PCR, respectively. The results showed that co-culture of HADSCs/HOBs, 1:1 grown into PHB–BHA promoted better cell adhesion, displayed a significant higher cell proliferation, higher production of ALP, extracellular mineralization and osteogenic-related gene expression of run-related transcription factor, bone sialoprotein, osteopontin, and osteocalcin compared to other coculture groups. This result also suggests that the use of electrospun PHB–BHA in a co-culture HADSCs/HOBs system may serve as promising approach to facilitate osteogenic differentiation activity of HADSCs through direct cell-to-cell contact with HOBs. Wiley 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47514/7/JBMA_35573.pdf application/pdf en http://irep.iium.edu.my/47514/10/47514_Differential%20osteogenic%20potential_WOS_SCOPUS.pdf Rozila, Ismail and Azari, Pedram and Munirah, Sha'ban and Wan Safwani, Wan Kamarul Zaman and Gan, Seng Neon and Nur Azurah, Abdul Ghani and Jahendran, Jeevanan and Pingguan-Murphy, Belinda and Chua, Kien Hui (2015) Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds. Journal of Biomedical Materials Research Part A, 104 (2). pp. 377-387. ISSN 15493296 http://dx.doi.org/10.1002/jbm.a.35573 10.1002/jbm.a.35573 |
| spellingShingle | R Medicine (General) Rozila, Ismail Azari, Pedram Munirah, Sha'ban Wan Safwani, Wan Kamarul Zaman Gan, Seng Neon Nur Azurah, Abdul Ghani Jahendran, Jeevanan Pingguan-Murphy, Belinda Chua, Kien Hui Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds |
| title | Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds |
| title_full | Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds |
| title_fullStr | Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds |
| title_full_unstemmed | Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds |
| title_short | Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds |
| title_sort | differential osteogenic potential of human adipose derived stem cells co cultured with human osteoblasts on polymeric microfiber scaffolds |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/47514/7/JBMA_35573.pdf http://irep.iium.edu.my/47514/10/47514_Differential%20osteogenic%20potential_WOS_SCOPUS.pdf |
| work_keys_str_mv | AT rozilaismail differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds AT azaripedram differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds AT munirahshaban differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds AT wansafwaniwankamarulzaman differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds AT gansengneon differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds AT nurazurahabdulghani differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds AT jahendranjeevanan differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds AT pingguanmurphybelinda differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds AT chuakienhui differentialosteogenicpotentialofhumanadiposederivedstemcellscoculturedwithhumanosteoblastsonpolymericmicrofiberscaffolds |