Scaling-up recombinant enzyme fermentation
Bioreactor or fermenter is the heart of a process. Scaling up a bioreactor and maintaining the same optimized process in a recombinant enzyme production in a higher scale is an engineering challenge. Scale-up is about mixing and mass transfer, a combination of art and science. It is more to methodol...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Book Chapter |
| Language: | English |
| Published: |
Springer International Publishing
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47856/8/One-Kenobi.pdf |
| _version_ | 1825649360674750464 |
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| author | Azmi, Azlin Suhaida Sulaiman, Sarina Mohamed Azmin, Nor Fadhillah Ali, Fathilah |
| author2 | Amid, Azura |
| author_facet | Amid, Azura Azmi, Azlin Suhaida Sulaiman, Sarina Mohamed Azmin, Nor Fadhillah Ali, Fathilah |
| author_sort | Azmi, Azlin Suhaida |
| collection | IIUM |
| description | Bioreactor or fermenter is the heart of a process. Scaling up a bioreactor and maintaining the same optimized process in a recombinant enzyme production in a higher scale is an engineering challenge. Scale-up is about mixing and mass transfer, a combination of art and science. It is more to methodological approach with some combination of rules of thumb, experience and/or trial and error. This chapter presented several bioreactor or fermenter modes and types before encompasses four different scale-up strategies. The scale-up strategies related to mass transfer involving constants scale-up of; (i)volumetric transfer coefficient (KLa), (ii) power input per liquid volume (P/V), (iii) impeller tip speed (Vi), and (iv) mixing time (t m) are described and discussed. |
| first_indexed | 2024-03-05T23:46:28Z |
| format | Book Chapter |
| id | oai:generic.eprints.org:47856 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T23:46:28Z |
| publishDate | 2015 |
| publisher | Springer International Publishing |
| record_format | dspace |
| spelling | oai:generic.eprints.org:478562016-07-19T13:09:52Z http://irep.iium.edu.my/47856/ Scaling-up recombinant enzyme fermentation Azmi, Azlin Suhaida Sulaiman, Sarina Mohamed Azmin, Nor Fadhillah Ali, Fathilah TA164 Bioengineering Bioreactor or fermenter is the heart of a process. Scaling up a bioreactor and maintaining the same optimized process in a recombinant enzyme production in a higher scale is an engineering challenge. Scale-up is about mixing and mass transfer, a combination of art and science. It is more to methodological approach with some combination of rules of thumb, experience and/or trial and error. This chapter presented several bioreactor or fermenter modes and types before encompasses four different scale-up strategies. The scale-up strategies related to mass transfer involving constants scale-up of; (i)volumetric transfer coefficient (KLa), (ii) power input per liquid volume (P/V), (iii) impeller tip speed (Vi), and (iv) mixing time (t m) are described and discussed. Springer International Publishing Amid, Azura 2015 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/47856/8/One-Kenobi.pdf Azmi, Azlin Suhaida and Sulaiman, Sarina and Mohamed Azmin, Nor Fadhillah and Ali, Fathilah (2015) Scaling-up recombinant enzyme fermentation. In: Recombinant Enzymes - From Basic Science to Commercialization. Springer International Publishing, Springer International Publishing Switzerland, pp. 99-113. ISBN 978-3-319-12397-4 http://link.springer.com/chapter/10.1007%2F978-3-319-12397-4_7 10.1007/978-3-319-12397-4_7 |
| spellingShingle | TA164 Bioengineering Azmi, Azlin Suhaida Sulaiman, Sarina Mohamed Azmin, Nor Fadhillah Ali, Fathilah Scaling-up recombinant enzyme fermentation |
| title | Scaling-up recombinant enzyme fermentation |
| title_full | Scaling-up recombinant enzyme fermentation |
| title_fullStr | Scaling-up recombinant enzyme fermentation |
| title_full_unstemmed | Scaling-up recombinant enzyme fermentation |
| title_short | Scaling-up recombinant enzyme fermentation |
| title_sort | scaling up recombinant enzyme fermentation |
| topic | TA164 Bioengineering |
| url | http://irep.iium.edu.my/47856/8/One-Kenobi.pdf |
| work_keys_str_mv | AT azmiazlinsuhaida scalinguprecombinantenzymefermentation AT sulaimansarina scalinguprecombinantenzymefermentation AT mohamedazminnorfadhillah scalinguprecombinantenzymefermentation AT alifathilah scalinguprecombinantenzymefermentation |