Production of cellulase from oil palm biomass as substrate by solid state bioconversion

Solid state bioconversion (SSB) of lignocellulosic material oil palm biomass (OPB) generated from palm oil industries as waste was conducted by evaluating the enzyme production through filamentous fungus in lab-scale experiment. OPB in the form of empty fruit bunches (EFB) was used as the solid subs...

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Main Authors: Alam, Md. Zahangir, Muhammad, Nurdina, Mahmat , Mohd Erman
Format: Article
Language:English
Published: Science Publications 2005
Subjects:
Online Access:http://irep.iium.edu.my/5030/1/Paper_AJAS.pdf
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author Alam, Md. Zahangir
Muhammad, Nurdina
Mahmat , Mohd Erman
author_facet Alam, Md. Zahangir
Muhammad, Nurdina
Mahmat , Mohd Erman
author_sort Alam, Md. Zahangir
collection IIUM
description Solid state bioconversion (SSB) of lignocellulosic material oil palm biomass (OPB) generated from palm oil industries as waste was conducted by evaluating the enzyme production through filamentous fungus in lab-scale experiment. OPB in the form of empty fruit bunches (EFB) was used as the solid substrate and treated with the fungus Trichoderma harzianum to produce cellulase. The results presented in this study revealed that the higher cellulase activity of 0.0413 unit was achieved at the day 3 of fermentation. While the optimum study indicated the enzyme production of 0.0433 unit with moisture content of 50%, 0.0413 unit with 5% v/w of inoculum size and 0.0413 unit with co-substrate concentration of 2% (w/w) at days 9, 9 and 12 of fungal treatment, respectively. The parameters glucosamine and reducing sugar were observed to evaluate the growth and substrate utilization in the experiment.
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spelling oai:generic.eprints.org:50302013-07-17T01:23:10Z http://irep.iium.edu.my/5030/ Production of cellulase from oil palm biomass as substrate by solid state bioconversion Alam, Md. Zahangir Muhammad, Nurdina Mahmat , Mohd Erman TP Chemical technology TP248.13 Biotechnology Solid state bioconversion (SSB) of lignocellulosic material oil palm biomass (OPB) generated from palm oil industries as waste was conducted by evaluating the enzyme production through filamentous fungus in lab-scale experiment. OPB in the form of empty fruit bunches (EFB) was used as the solid substrate and treated with the fungus Trichoderma harzianum to produce cellulase. The results presented in this study revealed that the higher cellulase activity of 0.0413 unit was achieved at the day 3 of fermentation. While the optimum study indicated the enzyme production of 0.0433 unit with moisture content of 50%, 0.0413 unit with 5% v/w of inoculum size and 0.0413 unit with co-substrate concentration of 2% (w/w) at days 9, 9 and 12 of fungal treatment, respectively. The parameters glucosamine and reducing sugar were observed to evaluate the growth and substrate utilization in the experiment. Science Publications 2005 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5030/1/Paper_AJAS.pdf Alam, Md. Zahangir and Muhammad, Nurdina and Mahmat , Mohd Erman (2005) Production of cellulase from oil palm biomass as substrate by solid state bioconversion. American Journal of Applied Sciences , 2 (2). pp. 569-572. ISSN 1546-9239 http://thescipub.com/abstract/10.3844/ajassp.2005.569.572 10.3844/ajassp.2005.569.572
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Alam, Md. Zahangir
Muhammad, Nurdina
Mahmat , Mohd Erman
Production of cellulase from oil palm biomass as substrate by solid state bioconversion
title Production of cellulase from oil palm biomass as substrate by solid state bioconversion
title_full Production of cellulase from oil palm biomass as substrate by solid state bioconversion
title_fullStr Production of cellulase from oil palm biomass as substrate by solid state bioconversion
title_full_unstemmed Production of cellulase from oil palm biomass as substrate by solid state bioconversion
title_short Production of cellulase from oil palm biomass as substrate by solid state bioconversion
title_sort production of cellulase from oil palm biomass as substrate by solid state bioconversion
topic TP Chemical technology
TP248.13 Biotechnology
url http://irep.iium.edu.my/5030/1/Paper_AJAS.pdf
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