Use of fungi for the bioconversion of rice straw into cellulase enzyme

Cellulase production was carried out by solid state bioconversion (SSB) method using rice straw, a lignocellulosic material and agricultural waste, as the substrate of three Trichoderma spp. and Phanerochaete chrysosporium in lab-scale experiments. The results were compared to select the best fungi...

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Main Authors: Khan, Munir H., Ali, S., Fakhru'l-Razi, A., Alam, Md. Zahangir
Format: Article
Language:English
Published: Taylor and Francis 2007
Subjects:
Online Access:http://irep.iium.edu.my/5039/1/Cellulase-munir.pdf
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author Khan, Munir H.
Ali, S.
Fakhru'l-Razi, A.
Alam, Md. Zahangir
author_facet Khan, Munir H.
Ali, S.
Fakhru'l-Razi, A.
Alam, Md. Zahangir
author_sort Khan, Munir H.
collection IIUM
description Cellulase production was carried out by solid state bioconversion (SSB) method using rice straw, a lignocellulosic material and agricultural waste, as the substrate of three Trichoderma spp. and Phanerochaete chrysosporium in lab-scale experiments. The results were compared to select the best fungi among them for the production of cellulase. Phanerochaete chrysosporium was found to be the best among these species of fungi, which produced the highest cellulase enzyme of 1.43 IU/mL of filter paper activity (FPase) and 2.40 IU/mL of carboxymethylcellulose activity (CMCase). The “glucosamine” and “reducing sugar” parameters were observed to evaluate the growth and substrate utilization in the experiments. In the case of Phanerochaete Chrysosporium, the highest glucosamine concentration was 1.60 g/L and a high concentration of the release of reducing sugar was measured as 2.58 g/L obtained on the 4th day of fermentation. The pH values were also recorded. The range of the pH was about 5.15 to 5.56 in the case of Phanerochaete Chrysosporium.
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spelling oai:generic.eprints.org:50392013-06-18T07:26:14Z http://irep.iium.edu.my/5039/ Use of fungi for the bioconversion of rice straw into cellulase enzyme Khan, Munir H. Ali, S. Fakhru'l-Razi, A. Alam, Md. Zahangir TP Chemical technology TP248.13 Biotechnology Cellulase production was carried out by solid state bioconversion (SSB) method using rice straw, a lignocellulosic material and agricultural waste, as the substrate of three Trichoderma spp. and Phanerochaete chrysosporium in lab-scale experiments. The results were compared to select the best fungi among them for the production of cellulase. Phanerochaete chrysosporium was found to be the best among these species of fungi, which produced the highest cellulase enzyme of 1.43 IU/mL of filter paper activity (FPase) and 2.40 IU/mL of carboxymethylcellulose activity (CMCase). The “glucosamine” and “reducing sugar” parameters were observed to evaluate the growth and substrate utilization in the experiments. In the case of Phanerochaete Chrysosporium, the highest glucosamine concentration was 1.60 g/L and a high concentration of the release of reducing sugar was measured as 2.58 g/L obtained on the 4th day of fermentation. The pH values were also recorded. The range of the pH was about 5.15 to 5.56 in the case of Phanerochaete Chrysosporium. Taylor and Francis 2007-05 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/5039/1/Cellulase-munir.pdf Khan, Munir H. and Ali, S. and Fakhru'l-Razi, A. and Alam, Md. Zahangir (2007) Use of fungi for the bioconversion of rice straw into cellulase enzyme. Journal of Environmental Science and Health, Part B, 42 (4). pp. 381-386. ISSN 0360-1234 http://dx.doi.org/10.1080/03601230701312647 doi:10.1080/03601230701312647
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Khan, Munir H.
Ali, S.
Fakhru'l-Razi, A.
Alam, Md. Zahangir
Use of fungi for the bioconversion of rice straw into cellulase enzyme
title Use of fungi for the bioconversion of rice straw into cellulase enzyme
title_full Use of fungi for the bioconversion of rice straw into cellulase enzyme
title_fullStr Use of fungi for the bioconversion of rice straw into cellulase enzyme
title_full_unstemmed Use of fungi for the bioconversion of rice straw into cellulase enzyme
title_short Use of fungi for the bioconversion of rice straw into cellulase enzyme
title_sort use of fungi for the bioconversion of rice straw into cellulase enzyme
topic TP Chemical technology
TP248.13 Biotechnology
url http://irep.iium.edu.my/5039/1/Cellulase-munir.pdf
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