Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge
A two-level fractional factorial design (FFD) was used to determine the effects of six factors, i.e. substrate (domestic wastewater sludge – DWS) and co-substrate concentration (wheat flour – WF), temperature, initial pH, inoculum size and agitation rate on the production of cellulase enzyme by Tric...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2008
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| Online Access: | http://irep.iium.edu.my/5041/1/Paper-2008-BT.pdf |
| _version_ | 1796875213228474368 |
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| author | Alam, Md. Zahangir Muyibi, Suleyman Aremu Wahid, Rosmaziah |
| author_facet | Alam, Md. Zahangir Muyibi, Suleyman Aremu Wahid, Rosmaziah |
| author_sort | Alam, Md. Zahangir |
| collection | IIUM |
| description | A two-level fractional factorial design (FFD) was used to determine the effects of six factors, i.e. substrate (domestic wastewater sludge – DWS) and co-substrate concentration (wheat flour – WF), temperature, initial pH, inoculum size and agitation rate on the production of cellulase enzyme by Trichoderma harzianum in liquid state bioconversion. On statistical analysis of the results from the experimental studies, optimum process conditions were found to be temperature 32.5 �C, substrate concentration (DWS) 0.75% (w/w), co-substrate (WF) concentration 2% (w/w), initial pH 5, inoculum size 2% (v/w) and agitation 175 rpm. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.975. Cellulase activity reached 10.2 FPU/ml at day 3 during the fermentation process which indicated about 1.5-fold increase in production compared to the cellulase activity obtained from the results of design of experiment (6.9 FPU/ml). Biodegradation of DWS was also evaluated to verify the efficiency of the bioconversion process as a waste
management method. |
| first_indexed | 2024-03-05T22:35:31Z |
| format | Article |
| id | oai:generic.eprints.org:5041 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:35:31Z |
| publishDate | 2008 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:generic.eprints.org:50412013-07-16T05:56:42Z http://irep.iium.edu.my/5041/ Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge Alam, Md. Zahangir Muyibi, Suleyman Aremu Wahid, Rosmaziah TP Chemical technology TP248.13 Biotechnology A two-level fractional factorial design (FFD) was used to determine the effects of six factors, i.e. substrate (domestic wastewater sludge – DWS) and co-substrate concentration (wheat flour – WF), temperature, initial pH, inoculum size and agitation rate on the production of cellulase enzyme by Trichoderma harzianum in liquid state bioconversion. On statistical analysis of the results from the experimental studies, optimum process conditions were found to be temperature 32.5 �C, substrate concentration (DWS) 0.75% (w/w), co-substrate (WF) concentration 2% (w/w), initial pH 5, inoculum size 2% (v/w) and agitation 175 rpm. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.975. Cellulase activity reached 10.2 FPU/ml at day 3 during the fermentation process which indicated about 1.5-fold increase in production compared to the cellulase activity obtained from the results of design of experiment (6.9 FPU/ml). Biodegradation of DWS was also evaluated to verify the efficiency of the bioconversion process as a waste management method. Elsevier 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5041/1/Paper-2008-BT.pdf Alam, Md. Zahangir and Muyibi, Suleyman Aremu and Wahid, Rosmaziah (2008) Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge. Bioresource Technology, 99 (11). pp. 4709-4716. ISSN 0960-8524 http://dx.doi.org/10.1016/j.biortech.2007.09.072 doi:10.1016/j.biortech.2007.09.072 |
| spellingShingle | TP Chemical technology TP248.13 Biotechnology Alam, Md. Zahangir Muyibi, Suleyman Aremu Wahid, Rosmaziah Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge |
| title | Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge |
| title_full | Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge |
| title_fullStr | Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge |
| title_full_unstemmed | Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge |
| title_short | Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge |
| title_sort | statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge |
| topic | TP Chemical technology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5041/1/Paper-2008-BT.pdf |
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