Management of palm oil mill effluent through production of cellulases by filamentous fungi

A laboratory scale study to evaluate the potentiality of filamentous fungi for the production of cellulolytic enzymes using palm oil mill effluent (POME)as a basal medium was initiated. A total of 25 filamentous fungi in which 16 filamentous fungi were isolated and purified from oil palm industria...

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Main Authors: Rashid, Shah Samiur, Alam, Md. Zahangir, Abdulkarim, Mohd Ismail, Mohd. Salleh, Hamzah
Format: Article
Language:English
Published: Springer 2009
Subjects:
Online Access:http://irep.iium.edu.my/5057/1/Cellulase-POME-screening.pdf
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author Rashid, Shah Samiur
Alam, Md. Zahangir
Abdulkarim, Mohd Ismail
Mohd. Salleh, Hamzah
author_facet Rashid, Shah Samiur
Alam, Md. Zahangir
Abdulkarim, Mohd Ismail
Mohd. Salleh, Hamzah
author_sort Rashid, Shah Samiur
collection IIUM
description A laboratory scale study to evaluate the potentiality of filamentous fungi for the production of cellulolytic enzymes using palm oil mill effluent (POME)as a basal medium was initiated. A total of 25 filamentous fungi in which 16 filamentous fungi were isolated and purified from oil palm industrial residues and 9 strains from laboratory stock were screened using POME with 1% total suspended solids. Trichoderma reesei RUT C-30 was identified as a potential strain for cellulolytic enzyme production as compared to other genera of Aspergillus, Penicillum, Rhizopus, Phanerochaete, Trichoderma and basidiomycete groups. The results showed that T. reesei RUT C-30 gave the highest filter paper cellulase and carboxy methyl cellulase activity of 0.917 and 2.51 U/ml respectively at day 5 of fermentation. Other parameters such as growth formation, pH, filterability and total biosolids were observed to evaluate the bioconversion process.
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spelling oai:generic.eprints.org:50572021-07-06T01:30:30Z http://irep.iium.edu.my/5057/ Management of palm oil mill effluent through production of cellulases by filamentous fungi Rashid, Shah Samiur Alam, Md. Zahangir Abdulkarim, Mohd Ismail Mohd. Salleh, Hamzah TP Chemical technology TP248.13 Biotechnology A laboratory scale study to evaluate the potentiality of filamentous fungi for the production of cellulolytic enzymes using palm oil mill effluent (POME)as a basal medium was initiated. A total of 25 filamentous fungi in which 16 filamentous fungi were isolated and purified from oil palm industrial residues and 9 strains from laboratory stock were screened using POME with 1% total suspended solids. Trichoderma reesei RUT C-30 was identified as a potential strain for cellulolytic enzyme production as compared to other genera of Aspergillus, Penicillum, Rhizopus, Phanerochaete, Trichoderma and basidiomycete groups. The results showed that T. reesei RUT C-30 gave the highest filter paper cellulase and carboxy methyl cellulase activity of 0.917 and 2.51 U/ml respectively at day 5 of fermentation. Other parameters such as growth formation, pH, filterability and total biosolids were observed to evaluate the bioconversion process. Springer 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5057/1/Cellulase-POME-screening.pdf Rashid, Shah Samiur and Alam, Md. Zahangir and Abdulkarim, Mohd Ismail and Mohd. Salleh, Hamzah (2009) Management of palm oil mill effluent through production of cellulases by filamentous fungi. World Journal of Microbiology and Biotechnology, 25 (12). pp. 2219-2226. ISSN 0959-3993 ; 1573-0972 http://dx.doi.org/10.1007/s11274-009-0129-9 doi:10.1007/s11274-009-0129-9
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Rashid, Shah Samiur
Alam, Md. Zahangir
Abdulkarim, Mohd Ismail
Mohd. Salleh, Hamzah
Management of palm oil mill effluent through production of cellulases by filamentous fungi
title Management of palm oil mill effluent through production of cellulases by filamentous fungi
title_full Management of palm oil mill effluent through production of cellulases by filamentous fungi
title_fullStr Management of palm oil mill effluent through production of cellulases by filamentous fungi
title_full_unstemmed Management of palm oil mill effluent through production of cellulases by filamentous fungi
title_short Management of palm oil mill effluent through production of cellulases by filamentous fungi
title_sort management of palm oil mill effluent through production of cellulases by filamentous fungi
topic TP Chemical technology
TP248.13 Biotechnology
url http://irep.iium.edu.my/5057/1/Cellulase-POME-screening.pdf
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