Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling
This work investigated the optimisation of the fermented culture medium for maximisation of rhamnolipids production produced by Pseudomonas aeruginosa 181 using Response Surface Modeling (RSM). A two full factorial central composite experimental design was used in the design of experiments and in th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer Berlin / Heidelberg
2007
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| Online Access: | http://irep.iium.edu.my/5233/1/fulltext.pdf |
| _version_ | 1825644639959384064 |
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| author | Al-Araji, Laith Issa Yassin Raja Abd Rahman, Raja Noor Zaliha Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Al-Araji, Laith Issa Yassin Raja Abd Rahman, Raja Noor Zaliha Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Al-Araji, Laith Issa Yassin |
| collection | IIUM |
| description | This work investigated the optimisation of the fermented culture medium for maximisation of rhamnolipids production produced by Pseudomonas aeruginosa 181 using Response Surface Modeling (RSM). A two full factorial central composite experimental design was used in the design of experiments and in the analysis of results. This procedure limited the number of actual experiments performed while allowing for possible interactions between the parameters (pH, stirring rate, casamino acid concentration and incubation period) on the production of biosurfactants. Production carried out at larger volumes of one litre using Bioreactor under RSM-optimised conditions yielded 3.61 g l−1 of products after purification by acid precipitation. |
| first_indexed | 2024-03-05T22:35:46Z |
| format | Article |
| id | oai:generic.eprints.org:5233 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:35:46Z |
| publishDate | 2007 |
| publisher | Springer Berlin / Heidelberg |
| record_format | dspace |
| spelling | oai:generic.eprints.org:52332011-11-29T04:09:00Z http://irep.iium.edu.my/5233/ Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling Al-Araji, Laith Issa Yassin Raja Abd Rahman, Raja Noor Zaliha Basri, Mahiran Salleh, Abu Bakar QR Microbiology TP248.13 Biotechnology This work investigated the optimisation of the fermented culture medium for maximisation of rhamnolipids production produced by Pseudomonas aeruginosa 181 using Response Surface Modeling (RSM). A two full factorial central composite experimental design was used in the design of experiments and in the analysis of results. This procedure limited the number of actual experiments performed while allowing for possible interactions between the parameters (pH, stirring rate, casamino acid concentration and incubation period) on the production of biosurfactants. Production carried out at larger volumes of one litre using Bioreactor under RSM-optimised conditions yielded 3.61 g l−1 of products after purification by acid precipitation. Springer Berlin / Heidelberg 2007-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5233/1/fulltext.pdf Al-Araji, Laith Issa Yassin and Raja Abd Rahman, Raja Noor Zaliha and Basri, Mahiran and Salleh, Abu Bakar (2007) Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling. Annals of Microbiology, 57 (4). pp. 571-575. ISSN 1590-4261 http://www.springerlink.com/content/1590-4261/ 10.1007/BF03175357 |
| spellingShingle | QR Microbiology TP248.13 Biotechnology Al-Araji, Laith Issa Yassin Raja Abd Rahman, Raja Noor Zaliha Basri, Mahiran Salleh, Abu Bakar Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling |
| title | Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling |
| title_full | Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling |
| title_fullStr | Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling |
| title_full_unstemmed | Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling |
| title_short | Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling |
| title_sort | optimisation of rhamnolipids produced by pseudomonas aeruginosa 181 using response surface modeling |
| topic | QR Microbiology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5233/1/fulltext.pdf |
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