| Summary: | Potential fungal strains belonging to the genera of Penicillium, Trichoderma and Aspergillus were isolated from various waste sources and tested for their capability to produce cellulase enzyme using sewage treatment plant (STP) sludge as a major substrate. Out of 35 strains, five strains were identified as the potential strains to produce cellulase through filter paper activity (FPA) assay. These five promising strains were TH(U), S-105A and P2-STP isolated from STP sludge, P1-EFB isolated from EFB compost, and O-102A isolated from rotten orange. Various carbon sources; wheat flour, cassava flour, commercial sugar and cellulose were evaluated as a co-substrate with and without EFB supplement using these five potential strains. The strain O-102A was identified as the most promising strain that able to produce cellulase enzyme using STP sludge as a major substrate, cellulose as a co-substrate with supplement of EFB.
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