Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate

Potential fungal strains belonging to the genera of Penicillium, Trichoderma and Aspergillus were isolated from various waste sources and tested for their capability to produce cellulase enzyme using sewage treatment plant (STP) sludge as a major substrate. Out of 35 strains, five strains were ident...

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Main Authors: Drani , Afifah, Alam, Md. Zahangir, Muyibi, Suleyman Aremu
Format: Article
Language:English
Published: Asian Network for Scientific Information 2011
Subjects:
Online Access:http://irep.iium.edu.my/5448/1/Cellulase-screening.pdf
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author Drani , Afifah
Alam, Md. Zahangir
Muyibi, Suleyman Aremu
author_facet Drani , Afifah
Alam, Md. Zahangir
Muyibi, Suleyman Aremu
author_sort Drani , Afifah
collection IIUM
description Potential fungal strains belonging to the genera of Penicillium, Trichoderma and Aspergillus were isolated from various waste sources and tested for their capability to produce cellulase enzyme using sewage treatment plant (STP) sludge as a major substrate. Out of 35 strains, five strains were identified as the potential strains to produce cellulase through filter paper activity (FPA) assay. These five promising strains were TH(U), S-105A and P2-STP isolated from STP sludge, P1-EFB isolated from EFB compost, and O-102A isolated from rotten orange. Various carbon sources; wheat flour, cassava flour, commercial sugar and cellulose were evaluated as a co-substrate with and without EFB supplement using these five potential strains. The strain O-102A was identified as the most promising strain that able to produce cellulase enzyme using STP sludge as a major substrate, cellulose as a co-substrate with supplement of EFB.
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spelling oai:generic.eprints.org:54482011-11-22T03:41:29Z http://irep.iium.edu.my/5448/ Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu TP248.13 Biotechnology Potential fungal strains belonging to the genera of Penicillium, Trichoderma and Aspergillus were isolated from various waste sources and tested for their capability to produce cellulase enzyme using sewage treatment plant (STP) sludge as a major substrate. Out of 35 strains, five strains were identified as the potential strains to produce cellulase through filter paper activity (FPA) assay. These five promising strains were TH(U), S-105A and P2-STP isolated from STP sludge, P1-EFB isolated from EFB compost, and O-102A isolated from rotten orange. Various carbon sources; wheat flour, cassava flour, commercial sugar and cellulose were evaluated as a co-substrate with and without EFB supplement using these five potential strains. The strain O-102A was identified as the most promising strain that able to produce cellulase enzyme using STP sludge as a major substrate, cellulose as a co-substrate with supplement of EFB. Asian Network for Scientific Information 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5448/1/Cellulase-screening.pdf Drani , Afifah and Alam, Md. Zahangir and Muyibi, Suleyman Aremu (2011) Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate. Biotechnology, 10 (2). pp. 197-202. ISSN 1682-2978 (O), 1682-296X (P) http://scialert.net/abstract/?doi=biotech.2011.197.202 DOI: 10.3923/biotech.2011.197.202
spellingShingle TP248.13 Biotechnology
Drani , Afifah
Alam, Md. Zahangir
Muyibi, Suleyman Aremu
Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate
title Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate
title_full Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate
title_fullStr Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate
title_full_unstemmed Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate
title_short Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate
title_sort screening of potential strains and co substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate
topic TP248.13 Biotechnology
url http://irep.iium.edu.my/5448/1/Cellulase-screening.pdf
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