Separation and purification of cellulase from fermentation broth of sewage sludge
In recent years, growing attention has been paid to the conversion of biomass into bioethanol which is considered the cleanest liquid fuel alternative to fossil fuels. One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce su...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
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2010
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| Online Access: | http://irep.iium.edu.my/5585/4/Separation_and_Purification_of_Cellulase.pdf http://irep.iium.edu.my/5585/1/purification_of_cellulase-conference.pdf |
| _version_ | 1796875277877379072 |
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| author | Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Mohd. Salleh, Hamzah Rashid , Shah Samiur |
| author_facet | Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Mohd. Salleh, Hamzah Rashid , Shah Samiur |
| author_sort | Mukhtaruddin, Muna Tasnim |
| collection | IIUM |
| description | In recent years, growing attention has been paid to the conversion of biomass into bioethanol which is considered the cleanest liquid fuel alternative to fossil fuels. One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce sugar which can be further fermented to produce ethanol. This study focuses on the purification of cellulases produced from fermentation broth of sewage sludge. In this study, crossflow filteration was used for the purification method using microfiltration (0.45 μm) and utrafiltration (10kD, 5 kD and 1 kD). The results revealed that ultrafiltration with membrane size of 1kD gave the best result for purification of cellulase from fermentation broth of sewage sludge which increases the enzyme activity from 22 U/ml after microfiltration to 50 U/ml after 1 kD of ultrafiltration. |
| first_indexed | 2024-03-05T22:36:27Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:5585 |
| institution | International Islamic University Malaysia |
| language | English English |
| last_indexed | 2024-03-05T22:36:27Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:55852021-06-23T03:06:09Z http://irep.iium.edu.my/5585/ Separation and purification of cellulase from fermentation broth of sewage sludge Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Mohd. Salleh, Hamzah Rashid , Shah Samiur TP Chemical technology TP248.13 Biotechnology In recent years, growing attention has been paid to the conversion of biomass into bioethanol which is considered the cleanest liquid fuel alternative to fossil fuels. One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce sugar which can be further fermented to produce ethanol. This study focuses on the purification of cellulases produced from fermentation broth of sewage sludge. In this study, crossflow filteration was used for the purification method using microfiltration (0.45 μm) and utrafiltration (10kD, 5 kD and 1 kD). The results revealed that ultrafiltration with membrane size of 1kD gave the best result for purification of cellulase from fermentation broth of sewage sludge which increases the enzyme activity from 22 U/ml after microfiltration to 50 U/ml after 1 kD of ultrafiltration. 2010 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/5585/4/Separation_and_Purification_of_Cellulase.pdf application/pdf en http://irep.iium.edu.my/5585/1/purification_of_cellulase-conference.pdf Mukhtaruddin, Muna Tasnim and Alam, Md. Zahangir and Mohd. Salleh, Hamzah and Rashid , Shah Samiur (2010) Separation and purification of cellulase from fermentation broth of sewage sludge. In: 2010 International Conference on Process Engineering and Advanced Materials (icpeam2010), 15th-17th June 2010, Kuala Lumpur Convention Centre, Kuala Lumpur. http://www.utp.edu.my/icpeam2010/ |
| spellingShingle | TP Chemical technology TP248.13 Biotechnology Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Mohd. Salleh, Hamzah Rashid , Shah Samiur Separation and purification of cellulase from fermentation broth of sewage sludge |
| title | Separation and purification of cellulase from fermentation broth of sewage sludge |
| title_full | Separation and purification of cellulase from fermentation broth of sewage sludge |
| title_fullStr | Separation and purification of cellulase from fermentation broth of sewage sludge |
| title_full_unstemmed | Separation and purification of cellulase from fermentation broth of sewage sludge |
| title_short | Separation and purification of cellulase from fermentation broth of sewage sludge |
| title_sort | separation and purification of cellulase from fermentation broth of sewage sludge |
| topic | TP Chemical technology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5585/4/Separation_and_Purification_of_Cellulase.pdf http://irep.iium.edu.my/5585/1/purification_of_cellulase-conference.pdf |
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